Difference between revisions of "User:Linh N Le/Notebook/2009/06/05"

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*making a new batch of MT's w/ Igor while Brigette  and Koch make antifade
*we need a new batch of brb80T, so we are mixing from stock at a 100:1 ratio of brb to T (thymine i think)
** [[User:Andy Maloney|Andy Maloney]] 00:01, 6 June 2009 (EDT): The "T" is for [http://en.wikipedia.org/wiki/Paclitaxel taxol]. Taxol is an anti-cancer drug that stabilizes microtubules.
*Since koch and brigette are still making antifade, igor and I used the old stuff from the other day
**We did not see anything, just blobs of dust
*Thinking I may have fried the tubes by stabilizing with cold brb80t, we look at a "naked" sample
**plenty of tubes, there must have been a misstep during the antifade application, or just bad antifade ate our tubes
*Igor and I try once more with the "Bad AF"
**We use 20ul of tubes (instead of 5 the first 2 times)
***We see plenty of tubes but the AF does not work, bleaches very fast
****Interesting note: we did see tubes break apart as they became bleached
****There was also a "flow" from the coverglass spreading out the liquid (i think Igor put more than 5ul of soln on there, but thats no big deal)
*Brigette finished her new batch of antifade and we try it with the new tubes
**After fussing around with focusing, she found the tubes and the antifade works great!
**Note: This sample has been the hardest for me to view. All the other samples this week were really easy to get in focus (kinda odd, who knows, maybe just a fluke)
**The tubes stay in view for a very long time and are quite contrastive
***Note: both brigette and I do not notice any movement of the tubes, normally there are some "wiggles" we see from loose ends
***They are stuck pretty hard on the glass and coverslide (as you can get a new set in view as you focus up the plane into the slide or coverglass)
**The new AF works like a charm, the images are lasting longer than any i have seen this week
**Brigette did a rough time in the bleaching and says they last ~1min 15s
===See also===
[[User:Steven J. Koch/Notebook/Kochlab/2009/06/05/New antifade components]]
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Revision as of 11:05, 29 September 2009

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