Difference between revisions of "User:Linh N Le/Notebook/2009/06/01"

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(Microtubules)
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==First Enty==
 
==First Enty==
 
*Koch had mentioned that I become more "science 2.0" and so I brought it upon myself to start a notebook here on the public wiki
 
*Koch had mentioned that I become more "science 2.0" and so I brought it upon myself to start a notebook here on the public wiki
==Microtubules==
+
==[[/Microtubules]]==
*We made MTs today
+
 
*First we took an aliquot (2ul) of FITC tubulin and warmed it in the PCR
 
**This caused the tubulin to polymerize into microtubules
 
**We also added a buffer, 198 ul of room temperature BRB80T, to stabilize them
 
*We then imaged them in the microscope
 
**We saw 100's of tubes (although they are smaller than what Koch made last week http://openwetware.org/wiki/User:Steven_J._Koch/Notebook/Kochlab/2009/05/26/FITC_Microtubules_with_antifade)
 
*We then made a batch with Antifade
 
**The old antifade from last week was no good, but a fresh batch came out great!
 
  
 
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Revision as of 10:02, 29 September 2009

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First Enty

  • Koch had mentioned that I become more "science 2.0" and so I brought it upon myself to start a notebook here on the public wiki

/Microtubules