Difference between revisions of "User:Le Borgne"

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==Reviews and Comments==
==Review articles and comments==
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Revision as of 22:23, 22 September 2010


Contact Info


Education and awards

  • 1995, PhD, EMBL-Heidelberg Germany
  • 1996-1999, post doctoral training at the Institut de Biology de Lille, France
  • 1999-2005, CNRS researcher at the ENS (Ecole Normale Supérieure), Paris, France
  • 2003 bronze medal from the CNRS
  • 2006 HDR, University de Rennes
  • 2006-present ATIP Programme CNRS, Institut de Génétique et Développement de Rennes, France

Research interests

Unequal segregation of Numb (red) in dividing SOP, tubulin (green)
  1. Asymmetric cell division is a conserved mechanism by which cell fate diversity is generated during Metazoan development. How one cell can generate two daughter cells with different identities and how defects in this asymmetry can contribute to cancer are the fundamental questions we are addressing in Drosophila. We are investigating this process in the context of asymmetric cell division of neural precursor cells, called Sensory Organ Precursor (SOP). These latter undergo four rounds of asymmetric divisions, in which mother cells generate distinct daughters via the unequal segregation of the cell-fate determinants Numb and Neuralized at mitosis. At each division binary cell fate decision are regulated by Delta-Notch dependent cell-cell signalling. Numb is an endocytic protein that can bind to Notch and a four pass transmembrane protein named Sanpodo (Spdo), a protein required for Notch activation in SOP lineage, thereby preventing Notch activation in this cell. Neur acts in SOPs and pIIb cells to regulate the endocytosis and signalling activity of Dl, thereby promoting N activation in non-SOP (lateral inhibition) and pIIa cells (binary cell fate decision), respectively. Despite intensive studies, the mechanism whereby Neur regulates Dl activity is not known. Two non-exclusive models have been proposed to explain the role of ubiquitin-dependent endocytosis of Dl in Notch receptor activation. First, the ‘pulling’ model proposes that internalization of Dl bound to its receptor exerts pulling forces on Notch and induces a conformational change exposing the S2 cleavage site to metalloproteases. Alternatively, the ‘activation/recycling’ model proposes that internalization is required to promote the formation of active ligands that are recycled back to the cell surface to activate Notch1. However the nature of the ligand activation and the subcellular localisation where recycled Dl could interact with Notch to produce signalling remained unknown. Our research aims to understand how intracellular trafficking contributes to the definition of the polarity axis of the divisions and ensure the proper spatio-temporal regulation of Notch-dependent fate decision.


Permanent positions

Lab Members early 2010
  • Roland Le Borgne, PI, DR2 CNRS
  • Solange Monier, CR1 INSERM
  • Stéphanie Le Bras, MCU, université deRennes 1

Postdoctoral Fellows

  • Christine Rondanino, INCA
  • Mathieu Cotton, UR1

Graduate Students

  • Najate Benhra, MNERT
  • Nabila Founounou, Ared Région Bretagne
  • Nicolas Loyer, MNERT

Technical Ingeneers and Research Technicians

  • Vignaux Françoise, I.E.1 CNRS

Past members

  • Sylvie Lallet Scientific Project manager at GNM healthcare consulting Group
  • Aurore Dussert =>Marek Mlodzik's Lab (in September 2010)

Scientific collaborations

Selected publications

  1. Le Borgne,R. (2006). Regulation of Notch signalling by endocytosis and endosomal sorting. Curr Opin Cell Biol. 18(2), 213-22

  2. Benhra N., Vignaux F., Dussert A., Schweisguth F. and Le Borgne R. (2010) Neuralized promotes basal to apical transcytosis of Delta in epithelial cells Molecular Biology of the Cell 21, 2078-86 (Cover Picture)

    Cover MBC Benhra et al, 2010low mag.jpg


  3. Le Borgne, R., Bardin, A. and Schweisguth F. (2005) Roles of receptor and ligand endocytosis in the regulation of Notch signaling. Development 132, 1751-62

  4. Dif A, Boulmedais F, Pinot M, Roullier V, Baudy-Floc'h M, Coquelle FM, Clarke S, Neveu P, Vignaux F, Le Borgne R, Dahan M, Gueroui Z, Marchi-Artzner V (2009) Small and stable peptidic PEGylated quantum dots to target polyhistidine-tagged proteins with controlled stoichiometry J. am. Chem. Soc. 131(41):14738-46.

  5. Bardin, A.J., Le Borgne, R., Schweisguth F. (2004). Asymmetric localization and function of cell-fate determinants: a fly's view. Curr Opin Neurobiol. Feb;14(1):6-14

  6. Smith CA, Lau KM, Rahmani Z, Dho SE, Brothers G, She YM, Berry DM, Bonneil E, Thibault P, Schweisguth F, Le Borgne R, McGlade CJ. (2007) aPKC-mediated phosphorylation regulates asymmetric membrane localization of the cell fate determinant Numb. EMBO J. 2007 Jan 24;26(2):468-80 F1000biology.gif

  7. Le Borgne, R. and Schweisguth, F. (2003). Notch signaling: endocytosis makes Delta signal better. Curr. Biol. 23, R273-R275

  8. Langevin,L., Le Borgne,R., (equal contribution), Rosenfeld, F., Gho,M., Schweisguth, F. and Bellaïche, Y. (2005). Lethal giant larvae controls the localisation of the Notch signalling regulators Numb, Neuralized and Sanpodo in Drosophila sensory organ precursor cells. Current Biology 15, 955-62

  9. Le Borgne R., Remaud, S., Hamel,S., and Schweisguth F (2005). The E3 ubiquitin ligases Mind bomb and Neuralized have distinct and complementary functions in the regulation of Delta and Serrate activity in drosophila. PLoS Biology 3, e96

  10. Le Borgne, R., and Hoflack, B. (1998). Protein transport from the secretory to the endocytic pathway in mammalian cells. Bioch. Biophys. Acta: thematic Issue on « The Golgi Complex », 1404: 195-209

  11. Le Borgne R. and Schweisguth, F. (2003). Unequal segregation of Neuralized biases Notch activation during asymmetric cell division. Developmental Cell 5(1), 139-148

  12. Le Borgne R., Bellaïche Y.and Schweisguth F.(2002) Drosophila E-Cadherin regulates the orientation of asymetric cell division in the sensory organ lineage, Current Biology, 12, 95-104

  13. Ben-Yaacov, S., Le Borgne, R., Abramson, I., Schweisguth,F. , and Schejter, E. D. (2001). Wasp, The Drosophila Wiskott-Aldrich Syndrome Gene Homolog, Is Required For Cell Fate Decisions Mediated By Notch Signaling . J. Cell Biol. 152, 1-13

  14. Waguri S., Dewitte F., Le Borgne R., Rouille Y., Uchiyama Y., Dubremetz JF, and Hoflack B. (2003). Visualization of TGN to Endosome Trafficking through Fluorescently Labeled MPR and AP-1 in Living Cells. Mol Biol Cell. 14(1):142-55

  15. Le Borgne R. and Hoflack B. (1998). Mechanisms of protein sorting and coat assembly: insights from the clathrin-coated vesicle pathway. Curr. Opin. Cell Biol. 10: 499-503

  16. Le Borgne R., Alconada A., Bauer U., and Hoflack B. (1998). The mammalian AP-3 adaptor-like complex mediates the intracellular transport of lysosomal membrane glycoproteins. J. Biol. Chem. 273: 29451-29461

  17. Le Borgne R., and Hoflack B. (1997). The mannose 6-phosphate receptors regulate the formation of clathrin-coated vesicles in the trans-Golgi network. J. Cell Biol. 137: 335-345

  18. Le Borgne R., Griffiths G., and Hoflack B. (1996). Mannose 6-phosphate receptors and ADP-ribosylation factors cooperate for high affinity interaction of the AP-1 Golgi-assembly proteins with membranes. J. Biol. Chem. 271: 2162-2170

  19. Le Borgne R., Schmidt A., Mauxion F., Griffiths G., and Hoflack B. (1993). Binding of AP-1 Golgi adaptors to membranes requires phosphorylated cytoplasmic domains of the mannose 6-phosphate/insulin-like growth factor II receptor. J. Biol. Chem. 268: 22552-22556


    Reviews and Comments[edit]


  20. Ludwig,T., Le Borgne,R., and Hoflack,B. (1995). Roles for mannose 6-phosphate receptors in lysosomal emzyme sorting, IGF-II binding and clathrin-coat assembly. Trends in Cell Biol. 5: 202-206


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