User:Klare Lazor/Notebook/Chem-496-001/2013/04/03: Difference between revisions
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==Description== | ==Description== | ||
#dye in dmso, add a little and mix with film in water | |||
#Add Cu(I) react for over 30 mins @ room temp | |||
#Cu(I) is unstable so add CuSO4 (Cu(II)) and ascorbic acid | |||
'''Dye Calculatations''' | |||
* 17.24µL butyne-1-ol in the hydrogel | |||
* (17.24/70.09)*.926= 2.08E-4 moles Butyne-1-ol | |||
*rxn is 1:1 however we want 1.5 * excess of dye | |||
*Dye= azide-flour 488: mw 563.35g/mol | |||
* 563.25g/mol * 2.28E-4moes = 1.28g * 1.5 =0.193 g | |||
**not enough dye so we cut off a portion of the hydrogel to use and dye | |||
'''Mass of cut hydrogel''' | |||
* mass of whole= 12.1419 g | |||
* mass of cut= 0.8810 | |||
* percent of whole = 7.26% | |||
*Original amount of dye needed= 0.014g | |||
** however, we only have 0.005 g | |||
'''Copper sulfat concentration''' | |||
*249.8*0.001moles/l*0.01l= 0.0025 g of copper sulfate | |||
*MADE A STOCK SOLUTION OF .025g/ml so 100µl =0.0025g of copper sulfate | |||
'''L-Ascorbic acid''' | |||
* 176.13g/mol * .1mol/L * 0.0L= 0.1761g | |||
*made stock solution of 1.761g per 10ml so 1 ml = .1761g | |||
''' | |||
range of dye volumes tested since did not have enough made a stock solution of 5 mg of dye in 1 ml of dmso''' | |||
==Data== | ==Data== |
Revision as of 08:14, 3 April 2013
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ObjectiveHydrogels: Copper catalyzed click rxn with azide flor Description
Dye Calculatations
Mass of cut hydrogel
Copper sulfat concentration
L-Ascorbic acid
range of dye volumes tested since did not have enough made a stock solution of 5 mg of dye in 1 ml of dmso Data
NotesThis area is for any observations or conclusions that you would like to note.
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