# Difference between revisions of "User:Klare Lazor/Notebook/Chem-496-001/2013/04/03"

Biomaterials Design Lab <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

## Objective

Hydrogels: Copper catalyzed click rxn with azide flor

## Description

1. dye in dmso, add a little and mix with film in water
2. Add Cu(I) react for over 30 mins @ room temp
3. Cu(I) is unstable so add CuSO4 (Cu(II)) and ascorbic acid

Dye Calculatations

• 17.24µL butyne-1-ol in the hydrogel
• rxn is 1:1 however we want 1.5 * excess of dye
• Dye= azide-flour 488: mw 563.35g/mol
• 563.25g/mol * 2.28E-4moes = 1.28g * 1.5 =0.193 g
• not enough dye so we cut off a portion of the hydrogel to use and dye

Mass of cut hydrogel

• mass of whole= 12.1419 g
• mass of cut= 0.8810
• percent of whole = 7.26%
• Original amount of dye needed= 0.014g
• however, we only have 0.005 g

Copper sulfat concentration

• 249.8*0.001moles/l*0.01l= 0.0025 g of copper sulfate
• MADE A STOCK SOLUTION OF .025g/ml so 100µl =0.0025g of copper sulfate

L-Ascorbic acid

• 176.13g/mol * .1mol/L * 0.0L= 0.1761g
• made stock solution of 1.761g per 10ml so 1 ml = .1761g

range of dye volumes tested since did not have enough made a stock solution of 5 mg of dye in 1 ml of dmso

## Data

• Add data and results here...

## Notes

This area is for any observations or conclusions that you would like to note.

Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.