User:Klare Lazor/Notebook/Chem-496-001/2013/04/03

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Hydrogels: Copper catalyzed click rxn with azide flor


  1. dye in dmso, add a little and mix with film in water
  2. Add Cu(I) react for over 30 mins @ room temp
  3. Cu(I) is unstable so add CuSO4 (Cu(II)) and ascorbic acid

Dye Calculatations

  • 17.24µL butyne-1-ol in the hydrogel
  • (17.24/70.09)*.926= 2.08E-4 moles Butyne-1-ol
  • rxn is 1:1 however we want 1.5 * excess of dye
  • Dye= azide-flour 488: mw 563.35g/mol
  • 563.25g/mol * 2.28E-4moes = 1.28g * 1.5 =0.193 g
    • not enough dye so we cut off a portion of the hydrogel to use and dye

Mass of cut hydrogel

  • mass of whole= 12.1419 g
  • mass of cut= 0.8810
  • percent of whole = 7.26%
  • Original amount of dye needed= 0.014g
    • however, we only have 0.005 g

Copper sulfat concentration

  • 249.8*0.001moles/l*0.01l= 0.0025 g of copper sulfate
  • MADE A STOCK SOLUTION OF .025g/ml so 100µl =0.0025g of copper sulfate

L-Ascorbic acid

  • 176.13g/mol * .1mol/L * 0.0L= 0.1761g
  • made stock solution of 1.761g per 10ml so 1 ml = .1761g

range of dye volumes tested since did not have enough made a stock solution of 5 mg of dye in 1 ml of dmso


  • Add data and results here...


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