User:Klare Lazor/Notebook/Chem-496-001/2013/03/11: Difference between revisions
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==Description== | ==Description== | ||
<u>Starter Culture Preparation</u> | |||
# Take out 4 aliquots of 4 mLs of broth from the 75 mL LB broth solution prepared last week and put into 4 test tubes | |||
# To each tube, add bacteria by scraping frozen bacteria (BL21 E. coli) with a micropipette tip (should be about 5 uL). Do this in a sterile environment (flame) | |||
# Place the tubes on a shaker at 37°C overnight. | |||
<u>Agar Plate Preparation</u> | |||
# Agar plate solution was prepared by adding 12.5 g LB and 10 g agar to 500 mL water. | |||
# The solution was autoclaved for one hour | |||
# Once the solution was cooled, 25 mL aliquots were poured into Petri dishes. | |||
#* This made about 20 agar plates | |||
# The plates were left in the fridge to set | |||
==Data== | ==Data== |
Revision as of 19:13, 26 March 2013
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ObjectiveDescriptionStarter Culture Preparation
Agar Plate Preparation
Data
NotesThis area is for any observations or conclusions that you would like to note.
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