- Create agar plates 20
- Started bacteria culture
- Autoclaved all solutions
- 8 Flask of 50mL of water and agar.
- Prepare LB in a 250mL Erlenmeyer flask
- 0.875g of LB
- 35mL of water
- Cover the flask with foil
- Allow the flask to cool
- Place the flask in a shaker/incubator and culture overnight at 37C and 200rpm
- Make certain that there is enough water in the reservoir before you begin.
- Turn the power on
- Turn the black knob to fill. This fills the chamber with water.
- Let water continue to fill the chamber until it reaches the line at the front of the chamber.
- Turn the black knob to STE (short for "sterilize").
- Load your media into the autoclave chamber. (Note: 2 2.8L Fernbach Flasks can be loaded into the chamber)
- Close the autoclave door and seal it shut.
- Set the temperature knob just to the right of 121F.
- Turn the timer knob to 50 minutes.
- After the 50 minutes have elapsed, wait 30 minutes until the pressure drops back to atmospheric pressure.
- Turn the black knob to Off.
- Use gloves to unload your sterilized material.
- Add data and results here...
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