User:Klare Lazor/Notebook/Chem-496-001/2013/03/04: Difference between revisions
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==Description== | ==Description== | ||
'''Autoclave''' | |||
*8 Flask of 50mL of water and agar. | |||
# Prepare [[AU_Biomaterials_Design_Lab:Materials/LB|LB]] in a 250mL Erlenmeyer flask | |||
# 0.875g of LB | |||
# 35mL of water | |||
# Cover the flask with foil | |||
# Autoclave | |||
# Allow the flask to cool | |||
# Place the flask in a shaker/incubator and culture overnight at 37C and 200rpm | |||
*Separate for starter culture | |||
*Make certain that there is enough water in the reservoir before you begin. | |||
# Turn the power on | |||
# Turn the black knob to fill. This fills the chamber with water. | |||
# Let water continue to fill the chamber until it reaches the line at the front of the chamber. | |||
# Turn the black knob to STE (short for "sterilize"). | |||
# Load your media into the autoclave chamber. (Note: 2 2.8L Fernbach Flasks can be loaded into the chamber) | |||
# Close the autoclave door and seal it shut. | |||
# Set the temperature knob just to the right of 121F. | |||
# Turn the timer knob to 50 minutes. | |||
# After the 50 minutes have elapsed, wait 30 minutes until the pressure drops back to atmospheric pressure. | |||
# Turn the black knob to Off. | |||
# Use gloves to unload your sterilized material. | |||
'''Starter Culture''' | |||
# Inoculate with the bacteria you are culturing using one of the two following methods | |||
# Using sterile methods, retrieve a single colony from a petrie dish and drop it into the flask | |||
# Place the flask in a shaker/incubator and culture overnight at 37C and 200rpm | |||
"made plates" | |||
==Data== | ==Data== |
Revision as of 12:05, 8 March 2013
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Objective
DescriptionAutoclave
Starter Culture
"made plates" Data
NotesThis area is for any observations or conclusions that you would like to note.
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