User:Klare Lazor/Notebook/Chem-496-001/2013/03/04: Difference between revisions
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'''Autoclave''' | '''Autoclave''' | ||
*8 Flask of 50mL of water and agar. | *8 Flask of 50mL of water and agar. | ||
# Prepare [[AU_Biomaterials_Design_Lab:Materials/LB|LB]] in a 250mL Erlenmeyer flask | |||
# 0.875g of LB | |||
# 35mL of water | |||
# Cover the flask with foil | |||
# Autoclave | |||
# Allow the flask to cool | |||
# Place the flask in a shaker/incubator and culture overnight at 37C and 200rpm | |||
*Make certain that there is enough water in the reservoir before you begin. | *Make certain that there is enough water in the reservoir before you begin. | ||
# Turn the power on | # Turn the power on |
Revision as of 11:56, 8 March 2013
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Objective
DescriptionAutoclave
Data
NotesThis area is for any observations or conclusions that you would like to note.
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