User:Klare Lazor/Notebook/Chem-496-001/2013/03/04: Difference between revisions
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==Description== | ==Description== | ||
'''Autoclave''' | |||
*Make certain that there is enough water in the reservoir before you begin. | |||
# Turn the power on | |||
# Turn the black knob to fill. This fills the chamber with water. | |||
# Let water continue to fill the chamber until it reaches the line at the front of the chamber. | |||
# Turn the black knob to STE (short for "sterilize"). | |||
# Load your media into the autoclave chamber. (Note: 2 2.8L Fernbach Flasks can be loaded into the chamber) | |||
# Close the autoclave door and seal it shut. | |||
# Set the temperature knob just to the right of 121F. | |||
# Turn the timer knob to 50 minutes. | |||
# After the 50 minutes have elapsed, wait 30 minutes until the pressure drops back to atmospheric pressure. | |||
# Turn the black knob to Off. | |||
# Use gloves to unload your sterilized material. | |||
==Data== | ==Data== |
Revision as of 11:54, 8 March 2013
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Objective
DescriptionAutoclave
Data
NotesThis area is for any observations or conclusions that you would like to note.
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