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Hydrogels for eventual attachment of protein

Revision as of 21:06, 27 February 2013

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Hydrogels for eventual attachment of protein


  • To eventually attach a protein, we can not crosslink in acidic or heated conditions. Therefore, for this procedure. We will be using hydrogels instead.
  • 4 hydrogels were prepared. 2 were made using PVOH MW = 22,000, while the other two were made using PVOH MW = 146,000 - 186,000. For each molecular weight, one hydrogel was made with isopropanol alone, and one was made with isopropanol + glutaraldehyde. The amounts of isopropanol used is the same as in previous trials.
  1. Measure out 2 samples of 1 g PVOH (MW = 22,000) and 2 samples of 1 g PVOH (MW = 146,000 - 186,000)
  2. Dissolve in 20 mL water (Dissolve on heat)
  3. Once dissolved, 0.0174 mL isopropanol was added to each sample. The samples were allowed to stir for a few minutes.
  4. 1 mL glutaraldehyde was added to the 2 hydrogels that are isopropanol + glut
  5. The hydrogels were placed in the freezer for 24 hours out for 4 hours in for 24hours and then out once again. repeated cycles.


  • Add data and results here...


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