# Difference between revisions of "User:Klare Lazor/Notebook/Chem-496-001/2013/02/01"

Biomaterials Design Lab <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

## Objective

1. Make Fixant for cross linking of the films on Monday
2. Make film with no glutaraldehyde (control)
3. Make film with glutaraldehyde no isopropyl alcohol (control)
4. Make five films varying in concentration of isopropyl alcohol, determine amount of alcohol by calculating amount of OH groups on PVA
5. Let all seven films dry over the weekend

## Description

Films and components All films were heated on a hot plate until the PVOH dissolved in the water. For films 5,6, and 7, additional water was added to the gel to assist in the dissolution. Once dissolved, the film was removed from heat and isopropyl and glutaraldehyde were added. The isopropyl was added first and stirred for roughly 2 to 3 minutes. Glutaraldehyde was added last.

1. 0.5043 g PVOH + 6 mL water (this was the only small film prepared)
2. 0.5027 g PVOH + 6 mL water + 1 mL glutaraldehyde
3. 0.5029 g PVOH + 6 mL water + 87 μL isopropyl stock solution + 1 mL glutaraldehyde
4. 0.5029 g PVOH + 6 mL water + 87 μL 10% isopropyl solution (see notes) + 1 mL glutaraldehyde
5. 0.5060 g PVOH + 6 mL water + 87 μL 1% isopropyl solution + 1 mL glutaraldehyde
6. 0.5034 g PVOH + 6 mL water + 87 μL 0.1% isopropyl solution + 1 mL glutaraldehyde
7. 0.5050 g PVOH + 6 mL water

isopropyl concentration determinations The concentrations determined to be tested were 0.1%, 1%, 10% and 100% isopropyl. Isopropanol solutions were pepared by taking 10 mL of isopropanol (see notes) and putting it in a tube. Serial dilutions with water were then carried out to create 10%, 1% and 0.1% solutions. To create these solutions, 1 mL of solution was added to 9 mL of water. 0.872 mL of each solution was added to its respective film.

## Data

• Add data and results here...

## Notes

This area is for any observations or conclusions that you would like to note.

Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.