User:Klare Lazor/Notebook/Chem-496-001/2012/11/14: Difference between revisions
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==Description== | ==Description== | ||
Prepare 3 PVOH/glutaraldehyde films, make another PVOH/Na-MT/glutaraldehyde film, attach a dye to the PVOH in a film, and prepare a solution of 0.01 M Na2SO4 | |||
Description | |||
PVOH/Glutaraldehyde Film Preparation | |||
#1 g PVOH was dissolved in 7 mL of water. | |||
#Once all the PVOH had dissolved, 1 mL of glutaraldehyde was added. | |||
#The solution was stirred for 5 minutes and then was poured into a dish and allowed to evaporate until Friday. | |||
PVOH/Na-MT/Glutaraldehyde Film Preparation | |||
#1 g PVOH and 0.1 g Na-MT were dissolved in 7 mL H2O on a heated hot plate. | |||
#Once the PVOH dissolved, 1 mL of glutaraldehyde was added. | |||
#The gel was stirred for 3 minutes and then was poured into a plastic dish and was allowed to evaporate until Friday. | |||
PVOH/Dye film preparation | |||
#Place 1 g PVOH in a small beaker | |||
#Dissolve in 7 mL of 0.1M NaHCO3/Na2CO3 buffer (pH=10) to deprotonate | |||
#Heat and stir to dissolve | |||
#Add 1 mg of dye (5-(4,6-Dichlorotiazinul Aminofluorescein) | |||
#Stir for 1 hr | |||
#Pour into dish | |||
0.01 M Na2SO4 Solution Preparation | |||
* 0.354 g Na2SO4 was added to 500 mL water in a volumetric flask. | |||
==Data== | ==Data== |
Revision as of 10:31, 19 November 2012
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ObjectiveDescriptionPrepare 3 PVOH/glutaraldehyde films, make another PVOH/Na-MT/glutaraldehyde film, attach a dye to the PVOH in a film, and prepare a solution of 0.01 M Na2SO4 Description PVOH/Glutaraldehyde Film Preparation
* 0.354 g Na2SO4 was added to 500 mL water in a volumetric flask. Data
NotesThis area is for any observations or conclusions that you would like to note.
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