Difference between revisions of "User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2013/04/23"

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(Autocreate 2013/04/23 Entry for User:Keyun_Wang/Notebook/Experimental_Biological_Chemistry_I)
 
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Experimental Biological Chemistry II</span>
 
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
 
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
 
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==Entry title==
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==Purpose==
* Insert content here...
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* DAY 7 - Continue running the 8-day study on cell growth in media containing films that has been soaking for 2 to 8 days.
  
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==Procedure==
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* Three flasks were taken out of the drawer: one flask containing silver-incorporated film, one containing control film, and one containing no film.
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* Starter pellet was taken out of freezer and defrosted in room temperature.
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* 3mL of LB were added to starter pellet and cells were resuspended.
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* 1mL of each resuspended cell culture was added into each flasks.
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* All flasks were placed in incubator shaker at 230rpm, 37°C, for 15 hours.
  
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==Observation==
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* The flasks containing the control films only appeared cloudy, indicating the flasks were contaminated.
 
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Revision as of 09:05, 30 April 2013

Owwnotebook icon.png Experimental Biological Chemistry II <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Purpose

  • DAY 7 - Continue running the 8-day study on cell growth in media containing films that has been soaking for 2 to 8 days.

Procedure

  • Three flasks were taken out of the drawer: one flask containing silver-incorporated film, one containing control film, and one containing no film.
  • Starter pellet was taken out of freezer and defrosted in room temperature.
  • 3mL of LB were added to starter pellet and cells were resuspended.
  • 1mL of each resuspended cell culture was added into each flasks.
  • All flasks were placed in incubator shaker at 230rpm, 37°C, for 15 hours.

Observation

  • The flasks containing the control films only appeared cloudy, indicating the flasks were contaminated.