Difference between revisions of "User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2013/04/17"

From OpenWetWare
Jump to: navigation, search
(Purpose)
(Purpose)
 
Line 7: Line 7:
 
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
 
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
 
==Purpose==
 
==Purpose==
* DAY 1 - Continue running the 8-day study on cell growth in media containing films that has been soaked for 2 to 8 days.
+
* DAY 1 - Continue running the 8-day study on cell growth in media containing films that has been soaking for 2 to 8 days.
  
 
==Procedure==
 
==Procedure==

Latest revision as of 00:06, 24 April 2013

Owwnotebook icon.png Experimental Biological Chemistry II <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>      </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Purpose

  • DAY 1 - Continue running the 8-day study on cell growth in media containing films that has been soaking for 2 to 8 days.

Procedure

  • Three flasks were taken out of the drawer: one flask containing silver-incorporated film, one containing control film, and one containing no film.
  • Starter pellet was taken out of freezer and defrosted in room temperature.
  • 3mL of LB were added to starter pellet and cells were resuspended.
  • 1mL of each resuspended cell culture was added into each flasks.
  • All flasks were placed in incubator shaker at 230rpm, 37°C, for 15 hours.