Difference between revisions of "User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2013/04/03"

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{|{{table}} width="800"
 
{|{{table}} width="800"
 
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Experimental Biological Chemistry</span>
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Experimental Biological Chemistry II</span>
 
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
 
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
 
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==Purpose==
 
==Purpose==
 
* Prepare films for cell growth study on Sunday.
 
* Prepare films for cell growth study on Sunday.
 +
* Prepare starter culture.
  
 
==Procedure==
 
==Procedure==
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* From the dried films, four pieces of 0.5g films in circular shapes were cut out from each film.
 
* From the dried films, four pieces of 0.5g films in circular shapes were cut out from each film.
 
* Films were placed in small plastic bag for storage.
 
* Films were placed in small plastic bag for storage.
 +
* Cells were harvested from shaker. The cells were placed in centrifuge to spin at 4°C, 4700rpm, for 30 minutes.
 +
* Supernatents were discarded for each falcon tube.
 +
* Pellets were placed in -80°C freezer until use.
  
  

Revision as of 19:55, 7 April 2013

Owwnotebook icon.png Experimental Biological Chemistry II <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Purpose

  • Prepare films for cell growth study on Sunday.
  • Prepare starter culture.

Procedure

  • AgLMT/polymer and LMT/polymer films were dried over night.
  • From the dried films, four pieces of 0.5g films in circular shapes were cut out from each film.
  • Films were placed in small plastic bag for storage.
  • Cells were harvested from shaker. The cells were placed in centrifuge to spin at 4°C, 4700rpm, for 30 minutes.
  • Supernatents were discarded for each falcon tube.
  • Pellets were placed in -80°C freezer until use.