User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2013/04/02

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Purpose

  • The purpose of the experiment today is to make starter culture in order to prepare for a 15-hour cell growth experiment tomorrow.

Procedure

  • 3mL of starter culture, each in 4 falcon tubes were made.
  • In 4 falcon tubes labeled "DH5α-T1 starter culture", 3mL of autoclaved LB were pipetted, respectively.
  • With a wooden stick, a portion of E.coli cells were picked from cell plates made on 02/12/2013 and dipped in one falcon tube containing 3mL of autoclaved LB.
  • The above procedure was repeated three more times.
  • Cultures were placed in shaker at 37°C, 230rpm for 2 hours.
  • After shaking, culture were placed in centrifuge at 4°C, 4700rpm, for 30 minutes.
  • Supernatants were discarded, and pellet were stored in -80°C until next day morning.