Difference between revisions of "User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2013/02/20"

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Experimental Biological Chemistry II</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
 
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==Entry title==
+
==Purpose==
* Insert content here...
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* To harvest starter culture for future use.
 +
* To test the concentration of silver present in a solution with silver films versus time.
 +
 
 +
==Procedure==
 +
* DH5α-T1 cells were harvested.
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* The absorbance of cells were taken at 600nm, the results are shown as follows:
 +
{| {{table}}
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| align="center" style="background:#f0f0f0;"|'''Cell Starter'''
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| align="center" style="background:#f0f0f0;"|'''Absorbance at 600nm'''
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|-
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| 1||1.927
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|-
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| 2||1.944
 +
|-
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| 3||1.939
 +
|-
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| 4||1.954
 +
|}
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* The cells were centrifuged at 4700rpm, 4°C, for 30 minutes.
 +
* Supernatants were poured out, leaving the pellets.
 +
* Pellets were stored in -80°C until needed.
 +
* Concentration of silver in 50mL of sterile water was tested via conductivity probe.
 +
* 50mL of sterile water was placed in 125mL Erlenmeyer flask to resemble conditions of antibacterial tests.
 +
* Newly synthesized silver-incorporated film was originally weighted out to be 0.97grams. The film was cut with scissors until its mass became 0.50grams.
 +
* 1mL of 5M of KNO<sub>3</sub> was added into 50mL of sterile water to serve as an ionic strength adjustor. 1mL of 5M KNO<sub>3</sub> was determined by 20uL of 5M KNO<sub>3</sub> per milliliter of solution.
 +
* Silver probe was inserted first into sterile water with KNO<sub>3</sub> to measure conductivity in units of mV. Then, 0.5g of silver film was placed in solution under occasional steering. Throughout the following four hours, the conductivity of solution was taken for every 30 minutes.
 +
* The solution was then placed in room temperature for over night, final conductivity will be taken the next day.
 +
* The concentration of silver, if all silver successfully leaked out into water, was calculated and shown in [[User:Melissa Novy/Notebook/CHEM-572/2013/02/20|Melissa's Notebook]]. The final concentration of silver in solution, if all silver were to leak out from the film, was calculated to be 23.8uM. The upcoming pilot study testing ideal concentration of silver in solution for best antibiotic results will have a range that include this concentration.
  
  

Latest revision as of 21:28, 26 September 2017

Owwnotebook icon.png Experimental Biological Chemistry II Report.pngMain project page
Resultset previous.pngPrevious entry      Next entryResultset next.png

Purpose

  • To harvest starter culture for future use.
  • To test the concentration of silver present in a solution with silver films versus time.

Procedure

  • DH5α-T1 cells were harvested.
  • The absorbance of cells were taken at 600nm, the results are shown as follows:
Cell Starter Absorbance at 600nm
1 1.927
2 1.944
3 1.939
4 1.954
  • The cells were centrifuged at 4700rpm, 4°C, for 30 minutes.
  • Supernatants were poured out, leaving the pellets.
  • Pellets were stored in -80°C until needed.
  • Concentration of silver in 50mL of sterile water was tested via conductivity probe.
  • 50mL of sterile water was placed in 125mL Erlenmeyer flask to resemble conditions of antibacterial tests.
  • Newly synthesized silver-incorporated film was originally weighted out to be 0.97grams. The film was cut with scissors until its mass became 0.50grams.
  • 1mL of 5M of KNO3 was added into 50mL of sterile water to serve as an ionic strength adjustor. 1mL of 5M KNO3 was determined by 20uL of 5M KNO3 per milliliter of solution.
  • Silver probe was inserted first into sterile water with KNO3 to measure conductivity in units of mV. Then, 0.5g of silver film was placed in solution under occasional steering. Throughout the following four hours, the conductivity of solution was taken for every 30 minutes.
  • The solution was then placed in room temperature for over night, final conductivity will be taken the next day.
  • The concentration of silver, if all silver successfully leaked out into water, was calculated and shown in Melissa's Notebook. The final concentration of silver in solution, if all silver were to leak out from the film, was calculated to be 23.8uM. The upcoming pilot study testing ideal concentration of silver in solution for best antibiotic results will have a range that include this concentration.