User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2013/02/19: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Experimental Biological Chemistry I</span> | ||
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | |style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | ||
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== | ==Purpose== | ||
* | * To make starter cultures of DH5α-T1 cells and autoclaved LB media for future use. | ||
==Procedure== | |||
* One colony of DH5α-T1 cell was inoculated with a sterile wooden stick and dipped into 3mL of sterile LB media inside a 15mL falcon tube. | |||
* This process was repeated three more times to obtain a total of four cell culture-containing falcon tubes. | |||
* The culture were placed in orbital shaker at 230rpm, at 37°C, for 15 hours. | |||
* 12.5g of LB media was weighted out and placed in a 250mL Erlenmeyer flask. | |||
* 50mL of distilled water was weighted out and poured into the Erlenmeyer flask. | |||
* This process was repeated five more times to obtain a total of six LB-containing flasks. | |||
* The flasks were placed in autoclave under liquid cycle. | |||
Revision as of 13:18, 19 February 2013
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Purpose
Procedure
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