Difference between revisions of "User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2013/02/14"

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(Autocreate 2013/02/14 Entry for User:Keyun_Wang/Notebook/Experimental_Biological_Chemistry_I)
 
 
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Experimental Biological Chemistry</span>
 
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
 
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
 
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==Entry title==
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==Purpose==
* Insert content here...
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* To harvest cells grown on [[User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2013/02/13|2013/02/13]] and store pellet in -80°C freezer for future use.
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==Procedure==
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* Cells were taken off the orbital shaker and were centrifuged at 4700rpm, at 4°C, for 30 minutes.
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* The supernatent was poured out, leaving the pellet alone.
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* The pellet was placed in -80°C freezer.
  
  

Latest revision as of 16:42, 18 February 2013

Owwnotebook icon.png Experimental Biological Chemistry <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>      </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Purpose

  • To harvest cells grown on 2013/02/13 and store pellet in -80°C freezer for future use.

Procedure

  • Cells were taken off the orbital shaker and were centrifuged at 4700rpm, at 4°C, for 30 minutes.
  • The supernatent was poured out, leaving the pellet alone.
  • The pellet was placed in -80°C freezer.