Difference between revisions of "User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2013/02/12"

From OpenWetWare
Jump to: navigation, search
(Autocreate 2013/02/12 Entry for User:Keyun_Wang/Notebook/Experimental_Biological_Chemistry_I)
 
(fix raw html notebook nav)
 
(One intermediate revision by one other user not shown)
Line 1: Line 1:
 
{|{{table}} width="800"
 
{|{{table}} width="800"
 
|-
 
|-
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
+
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Experimental Biological Chemistry II</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
+
|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
 
|-
 
|-
 
| colspan="2"|
 
| colspan="2"|
 
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
 
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
==Entry title==
+
==Purpose==
* Insert content here...
+
* Start DH5α-T1 cell growth on petri dish for future use.
  
 +
==Procedure==
 +
* Plates plated from [[User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2013/02/06|2013/02/06]] were transferred to fridge at 20°C due to neglect.
 +
* In order to ensure consistent cell growth for each experiment, a new dish was plated.
 +
* In a sterile environment, an autoclaved wooden stick was used to dip into the DH5α-T1 disk made on  [[User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2013/02/06|2013/02/06]] and strikes on a new LB+agar plate.
 +
* The dish was placed in -37°C for over night (16 hours).
  
 
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
 
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->

Latest revision as of 22:27, 26 September 2017

Owwnotebook icon.png Experimental Biological Chemistry II Report.pngMain project page
Resultset previous.pngPrevious entry      Next entryResultset next.png

Purpose

  • Start DH5α-T1 cell growth on petri dish for future use.

Procedure

  • Plates plated from 2013/02/06 were transferred to fridge at 20°C due to neglect.
  • In order to ensure consistent cell growth for each experiment, a new dish was plated.
  • In a sterile environment, an autoclaved wooden stick was used to dip into the DH5α-T1 disk made on 2013/02/06 and strikes on a new LB+agar plate.
  • The dish was placed in -37°C for over night (16 hours).