User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2013/02/05

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Purpose

  • Grind Ag+ Clay into powder for X-ray spectroscopic analysis.
  • Grow DH5α-T1 bacteria cells and make two glycerol stocks of it.

Procedure

  • Procedure for grinding of Ag+ Clay into powder form is recorded in Melissa's Notebook.
  • Growing of DH5α-T1 cells
    • One shot of DH5α-T1 cells was taken out from -87°C fridge.
    • Cells were thawed in room temperature
    • 3mL of autoclaved LB media was pipetted into a test tube
    • Inoculation loop was used to obtain a small portion of cells. The cells were then dipped into the 3mL LB media.
    • The cell culture was then placed in orbital shaker for 2 hours, at 37°C, with shaking at 230rpm.