User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2013/02/05: Difference between revisions

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Experimental Biological Chemistry II</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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==Entry title==
==Purpose==
* Insert content here...
* Grind Ag+ Clay into powder for X-ray spectroscopic analysis.
* Grow DH5α-T1 bacteria cells and make two glycerol stocks of it.
 
==Procedure==
* Procedure for grinding of Ag+ Clay into powder form is recorded in [[Melissa's Notebook]].
* Growing of DH5α-T1 cells
** One shot of DH5α-T1 cells was taken out from -87°C fridge.
** Cells were thawed in room temperature
** 3mL of autoclaved LB media was pipetted into a test tube
** Inoculation loop was used to obtain a small portion of cells. The cells were then dipped into the 3mL LB media.
** The cell culture was then placed in orbital shaker for 2 hours, at 37°C, with shaking at 230rpm.




Revision as of 12:01, 5 February 2013

Experimental Biological Chemistry II <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Purpose

  • Grind Ag+ Clay into powder for X-ray spectroscopic analysis.
  • Grow DH5α-T1 bacteria cells and make two glycerol stocks of it.

Procedure

  • Procedure for grinding of Ag+ Clay into powder form is recorded in Melissa's Notebook.
  • Growing of DH5α-T1 cells
    • One shot of DH5α-T1 cells was taken out from -87°C fridge.
    • Cells were thawed in room temperature
    • 3mL of autoclaved LB media was pipetted into a test tube
    • Inoculation loop was used to obtain a small portion of cells. The cells were then dipped into the 3mL LB media.
    • The cell culture was then placed in orbital shaker for 2 hours, at 37°C, with shaking at 230rpm.



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