Difference between revisions of "User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2013/02/05"

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(Autocreate 2013/02/05 Entry for User:Keyun_Wang/Notebook/Experimental_Biological_Chemistry_I)
 
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Experimental Biological Chemistry II</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
 
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==Entry title==
+
==Purpose==
* Insert content here...
+
* Grind Ag+ Clay into powder for X-ray spectroscopic analysis.
 +
* Grow DH5α-T1 bacteria cells and make two glycerol stocks of it.
 +
 
 +
==Procedure==
 +
* Procedure for grinding of Ag+ Clay into powder form is recorded in [[User:Melissa Novy/Notebook/CHEM-572/2013/02/05|Melissa's Notebook]].
 +
* Growing of DH5α-T1 cells
 +
** One shot of DH5α-T1 cells was taken out from -87°C fridge.
 +
** Cells were thawed in room temperature
 +
** 3mL of autoclaved LB media was pipetted into a test tube
 +
** Inoculation loop was used to obtain a small portion of cells. The cells were then dipped into the 3mL LB media.
 +
** The cell culture was then placed in orbital shaker for 2 hours, at 37°C, with shaking at 230rpm.
 +
** After two hours, the culture appeared clear, indicating no cell growth, an alternative approach were taken.
 +
* A different cell line was used for cell amplification, and the procedure for DH5α-T1 cell amplification was altered then repeated.
 +
** DH10B cells were taken from -87°C fridge.
 +
** The cell were let thaw on ice.
 +
** In two clean 15mL falcon tubes, 3mL autoclaved LB media were added.
 +
** In one falcon tube, 10uL DH10B cells were added.
 +
** In another falcon tube, 10uL DH5α-T1 cells were added.
 +
** The two falcon tubes were placed in orbital shaker for 2 hours, at 37°C, with shaking at 230rpm.
 +
** After 2 hours of incubation, the both cultures appeared clear. As a result, both cultures were placed in incubator horizontally to maximize shaking and left in shaker under same conditions for over night.
  
  

Latest revision as of 21:25, 26 September 2017

Owwnotebook icon.png Experimental Biological Chemistry II Report.pngMain project page
Resultset previous.pngPrevious entry      Next entryResultset next.png

Purpose

  • Grind Ag+ Clay into powder for X-ray spectroscopic analysis.
  • Grow DH5α-T1 bacteria cells and make two glycerol stocks of it.

Procedure

  • Procedure for grinding of Ag+ Clay into powder form is recorded in Melissa's Notebook.
  • Growing of DH5α-T1 cells
    • One shot of DH5α-T1 cells was taken out from -87°C fridge.
    • Cells were thawed in room temperature
    • 3mL of autoclaved LB media was pipetted into a test tube
    • Inoculation loop was used to obtain a small portion of cells. The cells were then dipped into the 3mL LB media.
    • The cell culture was then placed in orbital shaker for 2 hours, at 37°C, with shaking at 230rpm.
    • After two hours, the culture appeared clear, indicating no cell growth, an alternative approach were taken.
  • A different cell line was used for cell amplification, and the procedure for DH5α-T1 cell amplification was altered then repeated.
    • DH10B cells were taken from -87°C fridge.
    • The cell were let thaw on ice.
    • In two clean 15mL falcon tubes, 3mL autoclaved LB media were added.
    • In one falcon tube, 10uL DH10B cells were added.
    • In another falcon tube, 10uL DH5α-T1 cells were added.
    • The two falcon tubes were placed in orbital shaker for 2 hours, at 37°C, with shaking at 230rpm.
    • After 2 hours of incubation, the both cultures appeared clear. As a result, both cultures were placed in incubator horizontally to maximize shaking and left in shaker under same conditions for over night.