User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2013/01/30

From OpenWetWare
Owwnotebook icon.png Experimental Biological Chemistry II <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>


  • The purpose today is to autoclave LB medium to prepare for cell culture.
  • Also, Ag+ incorporated clay is going to be filtered with water and ethanol to isolate clay from water/ethanol solution.


  • 50mL LB media was made using the following way:
  0.50g Bacto-Trypsin
  0.25g Bacto-Yeast Extract
  0.50g Sodium Chloride
  50mL of distilled water
  Note: LB media obtained from Genova® with 2:1:2 proportion was used instead. 1.25g of LB powder was weighted out.
  • Each of the 50mL LB media was placed in two 125mL glass bottles and four 250mL Erlenmeyer flask.
  • 500mL of Agar was made using the following in a 1000mL Erlenmeyer flask:
  12.5g of LB media powder
  500mL of distilled water
  7.5g of Trypsin Soy Agar
  • The Erlenmeyer flask obtaining LB media or Agar were covered with aluminum foil, the bottles containing LB were capped loosely. All media were autoclaved under liquid cycle.
  • The clay+AgNO3 mixture was taken off hot plate.
  • A filter funnel, attached to 500mL Erlenmeyer flask then attached to a pump, is covered with a paper filter. Surface of paper filter was wetted and attached onto filter funnel through adding water.
  • The clay+AgNO3 mixture was then transfered into the filter funnel with suction applied.
  • Aluminum foil was used to cover the funnel to avoid silver to interact with light.


  • The clay+AgNO3 mixture when taken off the hot plate appeared black. However, during filtration, the filtered liquid solution remained clear in color.