User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/11/27: Difference between revisions
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* From the graph above, it could be suspected that there is a linear relationship between the mole ratio of Au to HRP in solution and the concentrations of gold nanoparticles in solution before protein aggregation takes over. | * From the graph above, it could be suspected that there is a linear relationship between the mole ratio of Au to HRP in solution and the concentrations of gold nanoparticles in solution before protein aggregation takes over. | ||
==Procedure for Running Atomic Absorption Spectrometer on Au/ADA and Au/HRP samples== | ==Procedure for Running AA Spectrometer on Au/ADA and Au/HRP samples== | ||
* Atomic Absorption spectra were first calibrated with HCl and gold solutions with the following range of concentrations: | |||
5ppm - 8ppm - 10ppm - 15ppm - 20ppm - 25ppm - 30ppm - 40ppm | |||
* Once the AA spectrometer was warmed up, the above HCl and gold solutions were run one by one for calibration. After the above eight solutions have ran, instrument was successfully calibrated. | |||
* Each Au/ADA and Au/HRP samples were injected into the AA spectrometer and ran individually. In between each run, the AA spectrometer inject tube was rinsed with distilled water. | |||
* The absorbance for Au/ADA and Au/HRP samples were recorded and converted to ppm. | |||
==Results for Running AA Spectrometer on Au/ADA and Au/HRP samples== | |||
* | |||
Revision as of 21:04, 27 November 2012
Experimental Biological Chemistry I | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Purpose
Procedure for Dialysis
Notes for Dialysis
Procedure for making dialyzed Au/ADA samples
60 - 70 - 80 - 90 - 100 - 110 - 120 - 130 - 140 - 150
Procedure for Running UV-vis Spectrometer on Au/ADA and Au/HRP samples
Results for Running UV-vis Spectrometer on Au/ADA and Au/HRP samples
Procedure for Running AA Spectrometer on Au/ADA and Au/HRP samples
5ppm - 8ppm - 10ppm - 15ppm - 20ppm - 25ppm - 30ppm - 40ppm
Results for Running AA Spectrometer on Au/ADA and Au/HRP samples
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