User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/10/02

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Purpose

  • Extract protein from cells as part of protein purification.

Procedure

  • Cells were taken out of -80C freezer and placed in ~30C warm water for cell thawing.
  • Once thawed, cells were sonicated and incubated on ice for each 30 second intervals. This process is repeated 3 times.
  • Once sonicated, cells were placed in centrifuge tubes and in centrifuge for 2 hours under 18,000 rpm and 4C.
  • Once centrifuged, supernatant were kept, filtered with 450mm filter paper, and kept in 4C until the next day.

Discussion