User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/10/02: Difference between revisions

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Experimental Biological Chemistry I</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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==Entry title==
==Purpose==
* Insert content here...
* Extract protein from cells as part of protein purification.
 
==Procedure==
* Cells were taken out of -80C freezer and placed in ~30C warm water for cell thawing.
* Once thawed, cells were sonicated and incubated on ice for each 30 second intervals. This process is repeated 3 times.
* Once sonicated, cells were placed in centrifuge tubes and in centrifuge for 2 hours under 18,000 rpm and 4C.
* Once centrifuged, supernatant were kept, filtered with 450mm filter paper, and kept in 4C until the next day.
 
==Discussion==
 




Revision as of 12:04, 3 October 2012

Experimental Biological Chemistry I <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Purpose

  • Extract protein from cells as part of protein purification.

Procedure

  • Cells were taken out of -80C freezer and placed in ~30C warm water for cell thawing.
  • Once thawed, cells were sonicated and incubated on ice for each 30 second intervals. This process is repeated 3 times.
  • Once sonicated, cells were placed in centrifuge tubes and in centrifuge for 2 hours under 18,000 rpm and 4C.
  • Once centrifuged, supernatant were kept, filtered with 450mm filter paper, and kept in 4C until the next day.

Discussion


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