Difference between revisions of "User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/09/04"

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==Entry title==
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==Purpose==
* Insert content here...
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* To run UV-Vis analysis on Au/BSA solutions at various ratios.  
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* To remake solutions that did not give expected results.
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* To make Tris Buffer at 100mM at pH 8.0 and 10.0
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==Procedure==
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* The absorbance of each sample...
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==Notes==
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* The original sets of solutions did not form nano-particles, solutions appeared yellow in color. It was predicted that the HAuCl4 reacted with gold specula during the transfer.
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* A new set of solutions with different Au/BSA ratios were made beforehand at the same ratio and concentrations. For details of the concentrations, see [[User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/08/29|entry from 2012/08/29]]
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* The new set of solutions with different ratios of Au and BSA appeared purpose in color. The 60 Au/BSA solution appeared cloudy and white. The 80 Au/BSA solution appeared as a purpose homogenous solution. The Au/BSA solution with other ratios are transparent with purpose fiber formations.  
  
  
 
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Revision as of 11:55, 4 September 2012

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Purpose

  • To run UV-Vis analysis on Au/BSA solutions at various ratios.
  • To remake solutions that did not give expected results.
  • To make Tris Buffer at 100mM at pH 8.0 and 10.0

Procedure

  • The absorbance of each sample...

Notes

  • The original sets of solutions did not form nano-particles, solutions appeared yellow in color. It was predicted that the HAuCl4 reacted with gold specula during the transfer.
  • A new set of solutions with different Au/BSA ratios were made beforehand at the same ratio and concentrations. For details of the concentrations, see entry from 2012/08/29
  • The new set of solutions with different ratios of Au and BSA appeared purpose in color. The 60 Au/BSA solution appeared cloudy and white. The 80 Au/BSA solution appeared as a purpose homogenous solution. The Au/BSA solution with other ratios are transparent with purpose fiber formations.