Difference between revisions of "User:Kersten S. Rabe"

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==Artwork==
 
==Artwork==
  
I worked a lot with the thermostable P450 CYP119A1,<ref>PMID: 18157853</ref> therefore I prepared a little movie for my PhD defence. [[Media:CYP119A1.ogg]]
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I worked a lot with the thermostable P450 CYP119A1,<ref>Rabe KS, Kiko K, and Niemeyer CM. Characterization of the peroxidase activity of CYP119, a thermostable P450 from Sulfolobus acidocaldarius. Chembiochem 2008 Feb 15; 9(3) 420-5. doi:10.1002/cbic.200700450 pmid:18157853.</ref> therefore I prepared a little movie for my PhD defence. [[Media:CYP119A1.ogg]]
 
In this movie you can see the P450 enzyme CYP119A1 with the inhibitor phenylimidazol bound to the active site.
 
In this movie you can see the P450 enzyme CYP119A1 with the inhibitor phenylimidazol bound to the active site.
  

Revision as of 02:54, 30 December 2009

Contact Info

Kersten S. Rabe - Half of him

I work as a Post-Doc in the Niemeyer lab at Dortmund University. In Dec. 2009 I finished my time as Doktorand with "summa cum laude" ("Ausgezeichnet")

Education

  • 2009-present Post-Doc with Prof. Niemeyer
  • 2005-2009 phD work in the group of Prof. Niemeyer at University of Dortmund, Germany, on Protein-DNA-conjugates. Title of thesis: "Herstellung rekombinanter Proteine für die Synthese von DNA-Protein-Konjugaten". Mark: summa cum laude ("Ausgezeichnet")
  • 2004 eight month research assistant at the group of Prof. Sprinzl, University of Bayreuth, Germany
  • 2004 Diploma (Biochemistry), Group of Prof. Sprinzl, University of Bayreuth, Germany
  • Research semester, Group of Dr. Ubbink, Protein Chemistry (former Metallo Protein Research), University of Leiden, Netherlands: "The Transient Complex of Cytochrome c and Cytochrome f - Investigated by Heteronuclear NMR"
  • 1999-2004, Graduate studies in Biochemistry, University of Bayreuth
  • 1999 Intermediate Diploma ("Vordiplom")
  • 1997-1999, Undergraduate studies in Biochemistry, University of Bayreuth
  • 1996/97 Thirteen month of civilian service
  • 1996 finished Abitur (final secondary school examination) at the Graf-Anton-Günther-Schule, Oldenburg, Germany

Research interests

  1. Synthetic biology
  2. Nanobiotechnology
  3. Chemical Biology

Artwork

I worked a lot with the thermostable P450 CYP119A1,<ref>Rabe KS, Kiko K, and Niemeyer CM. Characterization of the peroxidase activity of CYP119, a thermostable P450 from Sulfolobus acidocaldarius. Chembiochem 2008 Feb 15; 9(3) 420-5. doi:10.1002/cbic.200700450 pmid:18157853.</ref> therefore I prepared a little movie for my PhD defence. Media:CYP119A1.ogg In this movie you can see the P450 enzyme CYP119A1 with the inhibitor phenylimidazol bound to the active site.

Publications

First author

April 2009 I could design the coverpicture for ChemBioChem, where I could show my rendering skills again. [1]
  1. Rabe KS and Niemeyer CM. Hydrogels: Gene jelly. Nat Mater. 2009 May;8(5):370-2. DOI:10.1038/nmat2434 | PubMed ID:19387449 | HubMed [Paper1]
  2. Rabe KS, Spengler M, Erkelenz M, Müller J, Gandubert VJ, Hayen H, and Niemeyer CM. Screening for cytochrome p450 reactivity by harnessing catalase as reporter enzyme. Chembiochem. 2009 Mar 2;10(4):751-7. DOI:10.1002/cbic.200800750 | PubMed ID:19241405 | HubMed [Paper2]
  3. Rabe KS, Feldkamp U, and Niemeyer CM.

    Semisynthetic DNA-protein conjugates for fabrication of nucleic acid based nanostructures.

    AIP Conf. Proc. 2009; 1062(1) 19-32. doi: 10.1063/1.3012299 Permalink

    [Paper3]
  4. Rabe KS, Gandubert VJ, Spengler M, Erkelenz M, and Niemeyer CM. Engineering and assaying of cytochrome P450 biocatalysts. Anal Bioanal Chem. 2008 Nov;392(6):1059-73. DOI:10.1007/s00216-008-2248-9 | PubMed ID:18622752 | HubMed [Paper4]
  5. Rabe KS, Kiko K, and Niemeyer CM. Characterization of the peroxidase activity of CYP119, a thermostable P450 from Sulfolobus acidocaldarius. Chembiochem. 2008 Feb 15;9(3):420-5. DOI:10.1002/cbic.200700450 | PubMed ID:18157853 | HubMed [Paper5]
All Medline abstracts: PubMed | HubMed

Co-author

  1. Svahn MG, Rabe KS, Barger G, El-Andaloussi S, Simonson OE, Didier B, Olivier R, Dumy P, Brandén LJ, Niemeyer CM, and Smith CIE.

    High-Throughput Identification of Combinatorial Ligands for DNA Delivery in Cell Culture. AIP Conf. Proc. 2009; 1062(1) 73-88. doi: 10.1063/1.3012306 Permalink

    [Paper6]
  2. Agafonov DE, Rabe KS, Grote M, Voertler CS, and Sprinzl M. C-terminal modifications of a protein by UAG-encoded incorporation of puromycin during in vitro protein synthesis in the absence of release factor 1. Chembiochem. 2006 Feb;7(2):330-6. DOI:10.1002/cbic.200500358 | PubMed ID:16444758 | HubMed [Paper7]
  3. Agafonov DE, Rabe KS, Grote M, Huang Y, and Sprinzl M. The esterase from Alicyclobacillus acidocaldarius as a reporter enzyme and affinity tag for protein biosynthesis. FEBS Lett. 2005 Apr 11;579(10):2082-6. DOI:10.1016/j.febslet.2005.02.059 | PubMed ID:15811322 | HubMed [Paper8]
  4. Crowley PB, Rabe KS, Worrall JA, Canters GW, and Ubbink M. The ternary complex of cytochrome f and cytochrome c: identification of a second binding site and competition for plastocyanin binding. Chembiochem. 2002 Jun 3;3(6):526-33. DOI:10.1002/1439-7633(20020603)3:6<526::AID-CBIC526>3.0.CO;2-N | PubMed ID:12325008 | HubMed [Paper9]
All Medline abstracts: PubMed | HubMed

Patents

SPRINZL MATHIAS (DE); AGAFONOV DMITRY (DE); RABE KERSTEN (DE) ESTERASES FOR MONITORING PROTEIN BIOSYNTHESIS IN VITRO Publication number: WO2006082059

SPRINZL MATHIAS (DE); AGAFONOV DMITRY (DE); RABE KERSTEN (DE) CELL-FREE TRANSLATION SYSTEM FOR THE PRODUCTION OF (CHEMICALLY) MODIFIED PROTEINS Publication number: WO2006082058

References

  1. Rabe KS, Kiko K, and Niemeyer CM. Characterization of the peroxidase activity of CYP119, a thermostable P450 from Sulfolobus acidocaldarius. Chembiochem. 2008 Feb 15;9(3):420-5. DOI:10.1002/cbic.200700450 | PubMed ID:18157853 | HubMed [Rabe1]

Useful links