Difference between revisions of "User:Keoni K. Gandall/Notebook/Halobacterium NCR-1 vector"

From OpenWetWare
Jump to: navigation, search
(Project Description/Abstract)
(Project Description/Abstract)
Line 14: Line 14:
 
|colspan="2"|
 
|colspan="2"|
 
==Project Description/Abstract==
 
==Project Description/Abstract==
* I am trying to create a shuttle vector for Halobacterium NCR-1. To do this, I am synthesizing the HF2 origin of replication, then, using the gibson assembly, will put it into a custom vector as well, with pUC19 origin, chloramphenicol resistance gene, and the green fluorescent protein. This will answer a number of questions such as does the salt concentration in the media affect the GFP protein.  
+
* I am trying to create a shuttle vector for Halobacterium NCR-1. To do this, I am synthesizing the HF2 origin of replication, then, using the gibson assembly, will put it into a custom vector as well, with pUC19 origin, chloramphenicol resistance gene, and the green fluorescent protein. The first tests will be "does chloramphenicol affect Halobacterium?". If it does not, then I will just have to find another resistance gene. This will also answer a number of questions such as does the salt concentration in the media affect the GFP protein.  
  
* Lorem ipsum dolor sit amet, consectetuer adipiscing elit. Donec porta commodo tellus. Nam a est eget libero mollis tincidunt. Aliquam purus. Quisque nulla ligula, facilisis in, pulvinar sed, molestie a, quam. Vestibulum at pede. In in sem eget odio eleifend placerat. Phasellus ultricies felis quis sapien. Etiam molestie volutpat quam. Praesent pulvinar scelerisque mi. Nam mi urna, fringilla eu, mattis sed, venenatis id, nunc. Maecenas velit eros, congue ut, placerat in, ornare vel, sem. Aenean porta enim sit amet felis gravida posuere. Phasellus faucibus nibh et orci.
+
* Questions - Can chloramphenicol "kill" Halobacterium? Does the T7 promoter "work" in Halobacterium? Is the GFP protein affected by salt concentrations? How do we most efficiently transform Halobacterium NCR-1?
  
 
==Notes==
 
==Notes==

Revision as of 11:33, 31 December 2012

<!-- sibboleth --><div id="lncal1" style="border:0px;"><div style="display:none;" id="id">lncal1</div><div style="display:none;" id="dtext">12/31/2012,01/01/2013,01/02/2013,01/03/2013,01/07/2013,01/09/2013,01/10/2013,01/11/2013,01/12/2013,01/13/2013,01/15/2013,01/16/2013,01/17/2013,01/18/2013,01/19/2013,01/20/2013,01/21/2013,01/23/2013,01/24/2013,01/25/2013,01/26/2013,01/28/2013,01/29/2013,01/30/2013,01/31/2013,02/05/2013,02/06/2013,02/07/2013</div><div style="display:none;" id="page">User:Keoni K. Gandall/Notebook/Halobacterium NCR-1 vector</div><div style="display:none;" id="fmt">yyyy/MM/dd</div><div style="display:none;" id="css">OWWNB</div><div style="display:none;" id="month"></div><div style="display:none;" id="year"></div><div style="display:none;" id="readonly">Y</div></div>

Owwnotebook icon.png <sitesearch>title=Search this Project</sitesearch>

Customize your entry pages <html><img src="/images/a/aa/Help.png" border="0" /></html>

Project Description/Abstract

  • I am trying to create a shuttle vector for Halobacterium NCR-1. To do this, I am synthesizing the HF2 origin of replication, then, using the gibson assembly, will put it into a custom vector as well, with pUC19 origin, chloramphenicol resistance gene, and the green fluorescent protein. The first tests will be "does chloramphenicol affect Halobacterium?". If it does not, then I will just have to find another resistance gene. This will also answer a number of questions such as does the salt concentration in the media affect the GFP protein.
  • Questions - Can chloramphenicol "kill" Halobacterium? Does the T7 promoter "work" in Halobacterium? Is the GFP protein affected by salt concentrations? How do we most efficiently transform Halobacterium NCR-1?

Notes

  • Place some notes here for visitors
  • Example: This project is currently on hold until further notice.


Recent changes

19 October 2017

     21:07  BME100 f2017:Group7 W0800 L4‎ (diff | hist) . . (+977). . Miguel R. Almanza Lopez (talk | contribs) (SNP Information & Primer Design)

     18:43 

BME100 f2017:Group6 W1030 L4‎‎ (4 changes | history) . . (+1,447). . [Ray Gerard Regorgo‎ (4×)]

     

18:43

(cur | prev) . . (-196). . Ray Gerard Regorgo (talk | contribs) (OUR TEAM)

     

18:42

(cur | prev) . . (-3). . Ray Gerard Regorgo (talk | contribs) (OUR TEAM)

     

17:59

(cur | prev) . . (-3). . Ray Gerard Regorgo (talk | contribs) (Research and Development)

     

16:46

(cur | prev) . . (+1,649). . Ray Gerard Regorgo (talk | contribs) (Research and Development)

     15:43  User:Julie A. Fogarty‎ (diff | hist) . . (+46). . Julie A. Fogarty (talk | contribs) (Julie A. Fogarty)

     15:33 

Rich Lab:Funding‎‎ (2 changes | history) . . (-12). . [Isabel C. Morales‎ (2×)]

     

15:33

(cur | prev) . . (+12). . Isabel C. Morales (talk | contribs)

     

14:06

(cur | prev) . . (-24). . Isabel C. Morales (talk | contribs)

     14:58 

CONJ606:Materials‎‎ (2 changes | history) . . (+1,004). . [Weinjule‎; Peteral‎]

     

14:58

(cur | prev) . . (+685). . Peteral (talk | contribs) (Week 2: Me Myself and I-dentifiers, The Key To Immortality (Persistence))

     

12:15

(cur | prev) . . (+319). . Weinjule (talk | contribs) (Papers)

     14:52 

User:Peteral‎‎ (3 changes | history) . . (+1,264). . [Peteral‎ (3×)]

     

14:52

(cur | prev) . . (+185). . Peteral (talk | contribs) (Alec's Discussion Questions)

     

13:52

(cur | prev) . . (+351). . Peteral (talk | contribs) (Alec's Discussion Questions)

     

10:53

(cur | prev) . . (+728). . Peteral (talk | contribs) (Alec's Discussion Questions)

     14:13 

BME100 f2017:Group14 W0800 L4‎‎ (9 changes | history) . . (+9). . [Shae M. Diaz‎ (9×)]

     

14:13

(cur | prev) . . (+8). . Shae M. Diaz (talk | contribs) (Protocol)

     

14:12

(cur | prev) . . (-31). . Shae M. Diaz (talk | contribs) (TEAM MEMBERS)

     

14:10

(cur | prev) . . (+31). . Shae M. Diaz (talk | contribs) (TEAM MEMBERS)

     

14:09

(cur | prev) . . (+4). . Shae M. Diaz (talk | contribs) (TEAM MEMBERS)

     

14:05

(cur | prev) . . (-1). . Shae M. Diaz (talk | contribs) (TEAM MEMBERS)

     

14:05

(cur | prev) . . (+1). . Shae M. Diaz (talk | contribs) (TEAM MEMBERS)

     

14:04

(cur | prev) . . (-1). . Shae M. Diaz (talk | contribs) (TEAM MEMBERS)

     

13:55

(cur | prev) . . (+1). . Shae M. Diaz (talk | contribs) (TEAM MEMBERS)

     

13:55

(cur | prev) . . (-3). . Shae M. Diaz (talk | contribs) (TEAM MEMBERS)

 m   14:12  Swartz‎ (diff | hist) . . (+61). . Marcus Rohovie (talk | contribs)
     14:10 (Move log) . . Marcus Rohovie (talk | contribs) moved page Swartz to Swartz:Main

     14:08 

(Upload log). .

[Thorntca‎; Shae M. Diaz‎ (3×)]

     

14:08

. . Shae M. Diaz (talk | contribs) uploaded File:100profileshae.JPG

     

14:01

. . Shae M. Diaz (talk | contribs) uploaded File:Shaediaz100.JPG

     

13:51

. . Shae M. Diaz (talk | contribs) uploaded File:BME100ShaeD.JPG

     

13:17

. . Thorntca (talk | contribs) uploaded File:2Dgel.png.jpg

     13:52 

Klenow Assembly Method: Seamless cloning‎‎ (3 changes | history) . . (+101). . [David M.D. Bailey‎ (3×)]

     

13:52

(cur | prev) . . (-5). . David M.D. Bailey (talk | contribs) (The Klenow Assembly Method (KAM) for the seamless cloning of overlapping double stranded DNA fragments: Very cheap alternative to the Gibson Assembly)

     

13:51

(cur | prev) . . (+1). . David M.D. Bailey (talk | contribs) (The Klenow Assembly Method (KAM) for the seamless cloning of overlapping double stranded DNA fragments: Very cheap alternative to the Gibson Assembly)

     

13:50

(cur | prev) . . (+105). . David M.D. Bailey (talk | contribs) (The Klenow Assembly Method (KAM) for the seamless cloning of overlapping double stranded DNA fragments: Very cheap alternative to the Gibson Assembly)

     13:22 

User:Thorntca‎‎ (8 changes | history) . . (+529). . [Thorntca‎ (8×)]

     

13:22

(cur | prev) . . (+145). . Thorntca (talk | contribs)

     

13:19

(cur | prev) . . (+17). . Thorntca (talk | contribs)

     

13:19

(cur | prev) . . (+4). . Thorntca (talk | contribs)

     

13:18

(cur | prev) . . (+30). . Thorntca (talk | contribs)

     

13:16

(cur | prev) . . (+417). . Thorntca (talk | contribs)

     

12:52

(cur | prev) . . (+12). . Thorntca (talk | contribs)

     

12:51

(cur | prev) . . (-5). . Thorntca (talk | contribs)

     

12:51

(cur | prev) . . (-91). . Thorntca (talk | contribs)

     12:27 

User:Madeline.churchill‎‎ (2 changes | history) . . (+302). . [Madeline.churchill‎ (2×)]

     

12:27

(cur | prev) . . (0). . Madeline.churchill (talk | contribs) (TZAP protein controls telomere Trimming process)

     

12:27

(cur | prev) . . (+302). . Madeline.churchill (talk | contribs) (Madeline Churchill's Discussion Page)

     09:17  BioMicroCenter:BIG meeting‎ (diff | hist) . . (+19). . George W Bell (talk | contribs) (2017-2018 academic year)
     07:06 (User creation log) . . User account Newfish (talk | contribs) was created by Yar (talk | contribs) and password was sent by email ‎

     04:10 

BioMicroCenter:BioinfoClasses‎‎ (3 changes | history) . . (+132). . [Charlie Whittaker‎ (3×)]

     

04:10

(cur | prev) . . (+9). . Charlie Whittaker (talk | contribs) (Teaching Schedule)

     

04:09

(cur | prev) . . (0). . Charlie Whittaker (talk | contribs) (Teaching Schedule)

     

04:08

(cur | prev) . . (+123). . Charlie Whittaker (talk | contribs) (Teaching Schedule)

     03:36  User:Timothee Flutre‎ (diff | hist) . . (+146). . Timothee Flutre (talk | contribs) (add contrib breedR and SO)

 m   02:27 

How to write a research paper‎‎ (2 changes | history) . . (+91). . [Formand‎ (2×)]

 m   

02:27

(cur | prev) . . (-1). . Formand (talk | contribs) (External links)

 m   

02:24

(cur | prev) . . (+92). . Formand (talk | contribs) (External links)

18 October 2017

     19:09  BME100 f2017:Group10 W0800 L4‎ (diff | hist) . . (+1). . Osvaldo J Pagan (talk | contribs)