Difference between revisions of "User:Keoni K. Gandall/Notebook/Halobacterium NCR-1 vector"

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==Project Description/Abstract==
 
==Project Description/Abstract==
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*Goal- Create a plasmid for Halobacterium NCR-1 that the common DIY scientist can 1 obtain and 2 use
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* I am trying to create a shuttle vector for Halobacterium NCR-1. To do this, I am synthesizing the HF2 origin of replication, then, using the gibson assembly, will put it into a custom vector as well, with pUC19 origin, chloramphenicol resistance gene, and the green fluorescent protein. The first tests will be "does chloramphenicol affect Halobacterium?". If it does not, then I will just have to find another resistance gene. This will also answer a number of questions such as does the salt concentration in the media affect the GFP protein.  
 
* I am trying to create a shuttle vector for Halobacterium NCR-1. To do this, I am synthesizing the HF2 origin of replication, then, using the gibson assembly, will put it into a custom vector as well, with pUC19 origin, chloramphenicol resistance gene, and the green fluorescent protein. The first tests will be "does chloramphenicol affect Halobacterium?". If it does not, then I will just have to find another resistance gene. This will also answer a number of questions such as does the salt concentration in the media affect the GFP protein.  
  
* Questions - Can chloramphenicol "kill" Halobacterium? Does the T7 promoter "work" in Halobacterium? Is the GFP protein affected by salt concentrations? How do we most efficiently transform Halobacterium NCR-1? More to be answered...
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* Questions - Can chloramphenicol "kill" Halobacterium? Is the GFP protein affected by salt concentrations? How do we most efficiently transform Halobacterium NCR-1? More to be answered...
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*Change Log- Delete Does T7 promoter "work" in Halobacterium. No source of T7 polymerase gene.
  
 
==Notes==
 
==Notes==

Revision as of 20:21, 13 January 2013

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Project Description/Abstract

  • Goal- Create a plasmid for Halobacterium NCR-1 that the common DIY scientist can 1 obtain and 2 use


  • I am trying to create a shuttle vector for Halobacterium NCR-1. To do this, I am synthesizing the HF2 origin of replication, then, using the gibson assembly, will put it into a custom vector as well, with pUC19 origin, chloramphenicol resistance gene, and the green fluorescent protein. The first tests will be "does chloramphenicol affect Halobacterium?". If it does not, then I will just have to find another resistance gene. This will also answer a number of questions such as does the salt concentration in the media affect the GFP protein.
  • Questions - Can chloramphenicol "kill" Halobacterium? Is the GFP protein affected by salt concentrations? How do we most efficiently transform Halobacterium NCR-1? More to be answered...





  • Change Log- Delete Does T7 promoter "work" in Halobacterium. No source of T7 polymerase gene.

Notes

  • Place some notes here for visitors
  • Example: This project is currently on hold until further notice.


Recent changes

19 October 2017

     21:07  BME100 f2017:Group7 W0800 L4‎ (diff | hist) . . (+977). . Miguel R. Almanza Lopez (talk | contribs) (SNP Information & Primer Design)

     18:43 

BME100 f2017:Group6 W1030 L4‎‎ (4 changes | history) . . (+1,447). . [Ray Gerard Regorgo‎ (4×)]

     

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BME100 f2017:Group14 W0800 L4‎‎ (9 changes | history) . . (+9). . [Shae M. Diaz‎ (9×)]

     

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     13:52 

Klenow Assembly Method: Seamless cloning‎‎ (3 changes | history) . . (+101). . [David M.D. Bailey‎ (3×)]

     

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18 October 2017

     19:09  BME100 f2017:Group10 W0800 L4‎ (diff | hist) . . (+1). . Osvaldo J Pagan (talk | contribs)