User:Kelli B. Pointer
I am a new member of OpenWetWare!
Department of Biological Engineering
kpointer AT mit DOT edu
This website is for my 20.109 class, Laboratory Fundamentals of Biological Engineering.
Module 3 Project Proposal (work in progress)
Details and Methods
Blattmann, Annette et al., The formation of pores in the basal lamina of regenerated renal tubules Biomaterials (2008)
In this assay they looked at the basal aspects of renal tubules generated at the Interphase of an artificial interstitium in order to gain more knowledge about the generation of renal tubules. They took progenitor cells from neonatal rabbit kidney and put them inside a specific tissue holder that was covered by polyester fleece. They found tissue-specific antibodies that showed that the tubes were completely covered by a basal lamina. The matrix that was formed had three categories of pores which were widely distributed. The pores were also found in collections of duct tubules of the neonatal rabbit kidney.
Heydarkhan-Hagvall,Sepideh et al., Three-dimensional electrospun ECM-based hybrid scaffolds for cardiovascular tissue engineering, Biomaterials(2008)
Electrospinning can be used to take natural proteins or synthetic polymers to create fibrous scaffolds for tissue engineering. In order to try to overcome the problems of scaffolding that is electospun from natural proteins, in this assay they determined characteristics of a scaffold composed of collagen, elastin, and gelatin in order to avoid chemical cross-linking. They found that fiber size increased and pore size decreased when the polymer concentrations were increased. The tensile strength was less when compared to the traditional scaffolding that is made from natural proteins. Ultimately, they found that combining synthetic polymers and natural proteins to create a scaffold was biologically and mechanically favorable
- Stem Cells
- Tissue Engineering
Massachusetts Institute of Technology, 2010
Homewood-Flossmoor High School, 2006