User:Kathryn Muratore/Notebook/AU CHEM-570 lab prep/2011/08/24: Difference between revisions
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==Bench work== | ==Bench work== | ||
# PCR purification | # PCR purification | ||
* Followed instructions for Promega Gel and PCR purification kit, using spin column method | #* Followed instructions for Promega Gel and PCR purification kit, using spin column method | ||
* Purified experimental BSA PCR sample from [[../23| yesterday]] | #* Purified experimental BSA PCR sample from [[../23| yesterday]] | ||
# Double-digest | # Double-digest | ||
{| border="1" | {| border="1" | ||
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|0 μL ||5 μL ||0.5 μL ||39.5 μL from purified BSA PCR reaction in step 1 ||2.5μL ||2.5μL | |0 μL ||5 μL ||0.5 μL ||39.5 μL from purified BSA PCR reaction in step 1 ||2.5μL ||2.5μL | ||
|} | |} | ||
*2h @ 37°C | #*→2h @ 37°C | ||
#<li value="3">Gel purification | #<li value="3">Gel purification | ||
#*'''[[User:Kathryn Muratore|Kathryn Muratore]] 17:28, 24 August 2011 (EDT)''':I forgot to phosphatase the vectors before loading the gel, so we will phosphatase after purification. | #*'''[[User:Kathryn Muratore|Kathryn Muratore]] 17:28, 24 August 2011 (EDT)''':I forgot to phosphatase the vectors before loading the gel, so we will phosphatase after purification. | ||
Revision as of 14:32, 24 August 2011
 AU CHEM-570 Lab Prep
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ObjectiveRepeat BSA cloning into intein vectors with new, correct 3' primer. Bench work
Results
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