Difference between revisions of "User:Kathmc"

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== Bio ==
#REDIRECT [[Kathleen McGinness]]
*Postdoc in the [http://web.mit.edu/biology/sauerlab/ Sauer Lab] at [http://web.mit.edu/ MIT]
*Ph.D. in Chemistry, [http://www.scripps.edu/e_index.html The Scripps Research Institute], 2001 (with [http://www.scripps.edu/mb/joyce/joycelab.html Gerald Joyce])
*B.S. in Chemistry, [http://www.umd.edu University of Maryland College Park], 1996
*B.S. in Psychology, University of Maryland College Park, 1996
== Science stuff I know something about... ==
*''in vitro'' RNA techniques
*''in vitro'' selection and evolution of nucleic acids
*generation of nucleic acid and protein libraries
*nucleic acid-protein interactions
*fishing experiments to look for things that interact with your RNA/DNA/protein of interest
*PCR (even colony PCR, although it is '''not''' a separate skill)
*bacterial protein expression/purification
*''in vivo'' assays with ''E. coli'' i.e. analyzing effects on proteins/RNA
*molecular cloning
*Western/Northern/Southern blot analysis
*oligonucleotide synthesis
*RNA/DNA sequencing
*structural probing of RNA/DNA
*gels, all kinds of gels: agarose, native acrylamide,  denaturing acrylamide, SDS-acrylamide, two-dimensional
*enzyme kinetics
*radiolabeling nucleic acids and proteins
*tmRNA (or SsrA/10Sa RNA for you purists)
*energy-dependent proteolysis in bacteria
*The RNA World
*T7 RNA polymerase promoter preferences ''in vitro''
*molecular biology in a world without kits
My new favorite website detailing an alternative theory to evolution ([http://www.venganza.org/ WWFSMD?]).

Latest revision as of 20:25, 19 September 2005

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