Difference between revisions of "User:Karmella Haynes/Notebook/Polycomb project/2013/01/09"

From OpenWetWare
Jump to: navigation, search
(mm/dd/yy)
(01/09/13)
 
Line 13: Line 13:
 
'''Thaw cells'''<br>
 
'''Thaw cells'''<br>
 
* Quick thaw. Add to 7 mL FBS-tet-free
 
* Quick thaw. Add to 7 mL FBS-tet-free
* Spin for 5 min., 1000 rpm, r.t.
+
* Spin for 3 min., 1000 rpm, r.t.
 
** U2OS Flp-in TRex - reuspend in McCoy's 5A/ 10% FBS/ 1% p/s
 
** U2OS Flp-in TRex - reuspend in McCoy's 5A/ 10% FBS/ 1% p/s
 
** HEK293 Flp-in TRex - reuspend in DMEM / 10% FBS/ 1% p/s
 
** HEK293 Flp-in TRex - reuspend in DMEM / 10% FBS/ 1% p/s

Latest revision as of 19:29, 9 January 2013

Owwnotebook icon.png Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

01/09/13

  • Thaw new cells: U2OS Flp-in TRex, HEK293 Flp-in TRex

Thaw cells

  • Quick thaw. Add to 7 mL FBS-tet-free
  • Spin for 3 min., 1000 rpm, r.t.
    • U2OS Flp-in TRex - reuspend in McCoy's 5A/ 10% FBS/ 1% p/s
    • HEK293 Flp-in TRex - reuspend in DMEM / 10% FBS/ 1% p/s
  • Grow in small flat bottom flasks