Difference between revisions of "User:Karmella Haynes/Notebook/Polycomb project/2013/01/09"

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
 
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
 
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'''Thaw cells'''<br>
 
'''Thaw cells'''<br>
 
* Quick thaw. Add to 7 mL FBS-tet-free
 
* Quick thaw. Add to 7 mL FBS-tet-free
* Spin for 5 min., 1000 rpm, r.t.
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* Spin for 3 min., 1000 rpm, r.t.
 
** U2OS Flp-in TRex - reuspend in McCoy's 5A/ 10% FBS/ 1% p/s
 
** U2OS Flp-in TRex - reuspend in McCoy's 5A/ 10% FBS/ 1% p/s
 
** HEK293 Flp-in TRex - reuspend in DMEM / 10% FBS/ 1% p/s
 
** HEK293 Flp-in TRex - reuspend in DMEM / 10% FBS/ 1% p/s

Latest revision as of 21:20, 26 September 2017

Owwnotebook icon.png Project name Report.pngMain project page
Next entryResultset next.png

01/09/13

  • Thaw new cells: U2OS Flp-in TRex, HEK293 Flp-in TRex

Thaw cells

  • Quick thaw. Add to 7 mL FBS-tet-free
  • Spin for 3 min., 1000 rpm, r.t.
    • U2OS Flp-in TRex - reuspend in McCoy's 5A/ 10% FBS/ 1% p/s
    • HEK293 Flp-in TRex - reuspend in DMEM / 10% FBS/ 1% p/s
  • Grow in small flat bottom flasks