User:Karmella Haynes/Notebook/PcTF Genomics/2014/06/04: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">Pc-TF Genomics</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">Pc-TF Genomics</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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'''cDNA synthesis: K562'''<br> | '''cDNA synthesis: K562'''<br> | ||
* Use Carly's RNA from -80 °C | * Use Carly's RNA from -80 °C | ||
* | * K562 1 and 3 are +PcTF and K562 2 and 4 are controls (see [http://openwetware.org/wiki/User:Karmella_Haynes/Notebook/PcTF_Genomics/2012/12/18 12/18/12] ...will confirm when QC plate for mCh RT-PCR is run) | ||
* Will hold off on continuing U2OS for transiently transfected samples; will switch to KAH126 for further analyses | * Will hold off on continuing U2OS for transiently transfected samples; will switch to KAH126 for further analyses | ||
Latest revision as of 00:01, 27 September 2017
Pc-TF Genomics | Main project page Previous entry Next entry | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
06/04/14
cDNA synthesis: K562
--> Incubate at 65°C/ 5 min. Immediately place on ice for 1 min.
--> Aliquot 10 μL of mix into 8-tube strip
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