Difference between revisions of "User:Karmella Haynes/Notebook/PcTF Genomics/2014/02/14"

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(Autocreate 2014/02/14 Entry for User:Karmella_Haynes/Notebook/PcTF_Genomics)
 
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==02/14/14==
 
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* Line item 1
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* Miniprep digests: CMV/V0120, MV9
  
 
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'''Line item 1'''<br>
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'''Miniprep Digests'''<br>
> Samples
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# CMV culture 1 - check with E/P
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# CMV culture 2 - check with E/P
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# MV9 culture 1 - check with P/X
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# MV9 culture 2 - check with P/X
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* Used the Zymo miniprep kit
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* Pelleted each 5 mL culture at 2000 rpm/ 5 min in old centrifuge
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* Resuspended each pellet in 600 μL dH<sub>2</sub>O before miniprep
  
 
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Revision as of 14:35, 14 February 2014

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02/14/14

  • Miniprep digests: CMV/V0120, MV9

Miniprep Digests

  1. CMV culture 1 - check with E/P
  2. CMV culture 2 - check with E/P
  3. MV9 culture 1 - check with P/X
  4. MV9 culture 2 - check with P/X
  • Used the Zymo miniprep kit
  • Pelleted each 5 mL culture at 2000 rpm/ 5 min in old centrifuge
  • Resuspended each pellet in 600 μL dH2O before miniprep
Reagent Volume
reagent 1 # μL
reagent 2 #
reagent 3 #
reagent 4 #
dH2O #
  # μL

--> Reaction conditions