Difference between revisions of "User:Karmella Haynes/Notebook/PcTF Genomics/2014/01/27"

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{|
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| align="center" style="background:#f0f0f0;"|''''''
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| align="center" style="background:#f0f0f0;"|'''Template'''
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| align="center" style="background:#f0f0f0;"|'''Gene Target'''
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|-
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| Rxn 1:||treated cells||KIT, primers 13/14
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|-
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| Rxn 2:||treated cells||TNFRSF11A, primers 15/16
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|-
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| Rxn 3:||treated cells||EGFR, primers 17/18
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|-
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| Rxn 4:||treated cells||WT1, primers 19/20
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|-
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| Rxn 5:||treated cells||HLF, primer 21/22
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|-
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| Rxn 6:||treated cells||BCL6, primer 23/24
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|-
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| Rxn 7:||treated cells||mCh
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|-
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| Rxn 8:||treated cells||ref. gene, GAPD
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|-
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| Rxn 9:||untreated cells||KIT, primers 13/14
 +
|-
 +
| Rxn 10:||untreated cells||TNFRSF11A, primers 15/16
 +
|-
 +
| Rxn 11:||untreated cells||EGFR, primers 17/18
 +
|-
 +
| Rxn 12:||untreated cells||WT1, primers 19/20
 +
|-
 +
| Rxn 13:||untreated cells||HLF, primer 21/22
 +
|-
 +
| Rxn 14:||untreated cells||BCL6, primer 23/24
 +
|-
 +
| Rxn 15:||untreated cells||mCh
 +
|-
 +
| Rxn 16:||untreated cells||ref. gene, GAPD
 +
|-
 +
| Rxn 17:||no template||KIT, primers 13/14
 +
|-
 +
| Rxn 18:||no template||TNFRSF11A, primers 15/16
 +
|-
 +
| Rxn 19:||no template||EGFR, primers 17/18
 +
|-
 +
| Rxn 20:||no template||WT1, primers 19/20
 +
|-
 +
| Rxn 21:||no template||HLF, primer 21/22
 +
|-
 +
| Rxn 22:||no template||BCL6, primer 23/24
 +
|-
 +
| Rxn 23:||no template||mCh
 +
|-
 +
| Rxn 24:||no template||ref. gene, GAPD
 +
|}
  
  

Revision as of 16:45, 24 January 2014

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01/27/14

  • qRT-PCR experiment - PlateK562_1/ U2OS

qRT-PCR - Plate K562_1/ U2OS


' Template Gene Target
Rxn 1: treated cells KIT, primers 13/14
Rxn 2: treated cells TNFRSF11A, primers 15/16
Rxn 3: treated cells EGFR, primers 17/18
Rxn 4: treated cells WT1, primers 19/20
Rxn 5: treated cells HLF, primer 21/22
Rxn 6: treated cells BCL6, primer 23/24
Rxn 7: treated cells mCh
Rxn 8: treated cells ref. gene, GAPD
Rxn 9: untreated cells KIT, primers 13/14
Rxn 10: untreated cells TNFRSF11A, primers 15/16
Rxn 11: untreated cells EGFR, primers 17/18
Rxn 12: untreated cells WT1, primers 19/20
Rxn 13: untreated cells HLF, primer 21/22
Rxn 14: untreated cells BCL6, primer 23/24
Rxn 15: untreated cells mCh
Rxn 16: untreated cells ref. gene, GAPD
Rxn 17: no template KIT, primers 13/14
Rxn 18: no template TNFRSF11A, primers 15/16
Rxn 19: no template EGFR, primers 17/18
Rxn 20: no template WT1, primers 19/20
Rxn 21: no template HLF, primer 21/22
Rxn 22: no template BCL6, primer 23/24
Rxn 23: no template mCh
Rxn 24: no template ref. gene, GAPD