User:Karmella Haynes/Notebook/PcTF Genomics/2013/07/05: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
Line 11: Line 11:
<!-- Precede finished items with a checkmark &#x2713; -->
<!-- Precede finished items with a checkmark &#x2713; -->
* HEK293 Gal4-EED transfection - for PcTF expression and flow cytometry
* HEK293 Gal4-EED transfection - for PcTF expression and flow cytometry


----
----

Revision as of 20:22, 5 July 2013

Pc-TF Genomics <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>


07/05/13

  • HEK293 Gal4-EED transfection - for PcTF expression and flow cytometry


Lipo transfection
> Samples are for flow cytometry > Seed new 6-well plates w/ ~5x105 cells/well in 3.4 mL plain medium (don't have any p.s.-free)
> Transfect human PcTF under control of constitutive CMV-TetO promoter (KAH160/MV1); 347 ng/μL; Note: this cell line has TetR, so constitutive CMV is needed.


Wells Plasmid DNA Volume + dH2O Opti-MEM PLUS Lipo
1 KAH160/MV1 2 μg 8.8 μL +11.2 570 μL 2.5 μL 7.5 μL
2 KAH160/MV1 2 μg 8.8 μL +11.2 " " "
3 mock none 0 μL + 20.0 " " "
4 none none --- --- --- ---



Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Karmella_Haynes/Notebook/PcTF_Genomics/2013/07/05&oldid=707328"