User:Karmella Haynes/Notebook/PcTF Genomics/2013/07/04: Difference between revisions
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| sample 6 || 17,885 || x5 = || 89,425 | | sample 6 || 17,885 || x5 = || 89,425 | ||
|} | |} | ||
---- | |||
'''Gal4-EED/luc Dox induction #2'''<br> | |||
* Lower range worked very well for trail #1! Saw ranges in expression for 1.0 - 10<sup>-4</sup> μg/mL doc after 4 days | |||
* Repeat induction and test after 24 hours | |||
# 1.0 μg/mL dox | |||
# 10<sup>-1</sup> | |||
# 10<sup>-2</sup> | |||
# 10<sup>-3</sup> | |||
# 10<sup>-4</sup> | |||
# 0 | |||
Addition of serial dilutions of dox to cells | |||
* Start with 3.5 mL growth medium in cell culture wells. Remove some growth medium from cells where necessary. | |||
* Set up the following serial dilutions (using appropriate growth medium) in sterile 1 mL tubes... | |||
{| {{table}} | |||
|- | |||
| || tube 1 || 2 || 3 || 4 || 5 || 6 | |||
|- | |||
| 1 mg/mL dox (μL) || 8.0 || --- || --- || --- || --- || --- | |||
|- | |||
| mixture from prior tube (μL) || --- || 100 || 100 || 100 || 100 || --- | |||
|- | |||
| +growth med. (μL) || 1000 || 900 || 900 || 900 || 900 || --- | |||
|- | |||
| Final [dox] (μg/mL) in 4 mL med. || 1.0 || 10<sup>-1</sup> || 10<sup>-2</sup> || 10<sup>-3</sup> || 10<sup>-4</sup> || 0 | |||
|} | |||
Revision as of 21:11, 4 July 2013
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07/04/13
Luciferase activity assay - time point: 4 days
Gal4-EED/luc Dox induction #2
Addition of serial dilutions of dox to cells
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