User:Karmella Haynes/Notebook/PcTF Genomics/2013/06/30: Difference between revisions
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==6/30/13 | ==6/30/13== | ||
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* Gal4-EED/luc cells - split 6-well culture plate (shipment-2 cells) and treat 1 plate with dox | * Gal4-EED/luc cells - split 6-well culture plate (shipment-2 cells) and treat 1 plate with dox | ||
* PcTF transfection of SK-N-SH | * PcTF transfection of SK-N-SH | ||
---- | ---- | ||
'''Gal4-EED/luc cells | '''Gal4-EED/luc cells +dox'''<br> | ||
* Previous trial, saw no difference in repression for 0.1, 0.2, 0.5, 1.0, 2.0 μg/mL dox. Try lower range. | * Previous trial, saw no difference in repression for 0.1, 0.2, 0.5, 1.0, 2.0 μg/mL dox. Try lower range. | ||
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| 2 || KAH126/MV2 || 2 μg || 8.8 μL +11.2 || " || " || " | | 2 || KAH126/MV2 || 2 μg || 8.8 μL +11.2 || " || " || " | ||
|- | |- | ||
| 3 || <s>KAH126/MV2</s>* | | 3 || <s>KAH126/MV2</s>* || <s>2 μg</s> || <s>8.8 μL +11.2</s> || " || " || " | ||
|- | |- | ||
| 4 || mock || none || 0 μL + 20.0 || " || " || " | | 4 || mock || none || 0 μL + 20.0 || " || " || " | ||
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| 6 || mock || none || 0 μL + 20.0 || " || " || " | | 6 || mock || none || 0 μL + 20.0 || " || " || " | ||
|} | |} | ||
* Ran out of plasmid DNA | (*Ran out of plasmid DNA) | ||
> Add 570 μL Opti-MEM to each 20 μL DNA sample <br> | > Add 570 μL Opti-MEM to each 20 μL DNA sample <br> | ||
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> Grow cells at 37°C overnight<br> | > Grow cells at 37°C overnight<br> | ||
Results (7/01/13) | |||
* Checked RFP expression on the Nikon scope | |||
* Expression is good. Expand cells in 10 cm plates | |||
* Do TRIzol prep after ~10 days. | |||
Revision as of 20:12, 5 July 2013
Pc-TF Genomics | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6/30/13
Gal4-EED/luc cells +dox
Addition of serial dilutions of dox to cells
Lipo transfection
(*Ran out of plasmid DNA) > Add 570 μL Opti-MEM to each 20 μL DNA sample Results (7/01/13)
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