User:Karmella Haynes/Notebook/PcTF Genomics/2013/06/19: Difference between revisions
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''' | '''Luciferase activity assay - time point 2'''<br> | ||
> | |||
<u>Cell prep</u> | |||
* Harvested cells | |||
** Seeded new dox+ plate with 1/2 of cells | |||
** Pelleted other half, resuspended in 2 mL FACS buffer | |||
<u>Assay reagents</u> | |||
* Used the Biotium Steady-Luc Firefly HTS Assay Kit (same as 6/18/13) | |||
<u>Luc assay</u> | |||
* Filtered '''700 μL cells''' through strainer caps | |||
* Other steps same as 6/18/13 | |||
<u>Cell counts</u> | |||
* Wang lab's Accuri flow cytometer. | |||
* Set machine to read 20 uL of cells | |||
* Be sure to "clean" with water-run in between samples | |||
{| class="wikitable" border="0" cellspacing="3" <!-- Rxn. table --> | {| class="wikitable" border="0" cellspacing="3" <!-- Rxn. table --> | ||
|-valign="top" | |-valign="top" | ||
| <u> | | <u>Sample ID</u> || <u>Gated count/ 20 μL</u> || || <u>Cells/ 100 μL</u> | ||
|- | |- | ||
| | | sample 1 || || x5 = || | ||
|- | |- | ||
| | | sample 2 || || x5 = || | ||
|- | |- | ||
| | | sample 3 || || x5 = || | ||
|- | |- | ||
| | | sample 4 || || x5 = || | ||
|- | |- | ||
| | | sample 5 || || x5 = || | ||
|- | |- | ||
| | | sample 6 || || x5 = || | ||
|} | |} | ||
Revision as of 21:37, 19 June 2013
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mm/dd/yy
Luciferase activity assay - time point 2
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