Difference between revisions of "User:Karmella Haynes/Notebook/PcTF Genomics/2013/06/15"

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(06/15/13)
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# 0.5
 
# 0.5
 
# 1.0
 
# 1.0
# 2.0
+
# 2.0 (induced on 6/02/13, 13 days)
  
 
* Harvested cells from one 6-well plate (split plate #2 for further analyses)
 
* Harvested cells from one 6-well plate (split plate #2 for further analyses)

Revision as of 17:00, 17 June 2013

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06/15/13

  • Gal4-EED/luc Luciferase ICC - fixation, permeabilization, primary ab staining



Gal4-EED/luc Luciferase ICC

Samples (Shipment 1 cells)

  1. 0 μg/mL dox
  2. 0.1
  3. 0.2
  4. 0.5
  5. 1.0
  6. 2.0 (induced on 6/02/13, 13 days)
  • Harvested cells from one 6-well plate (split plate #2 for further analyses)
  • At the primary antibody staining step, keep samples and reagents on ice overnight in the cold room