Difference between revisions of "User:Karmella Haynes/Notebook/PcTF Genomics/2013/06/15"

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(06/15/13)
(06/15/13)
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<!-- Precede finished items with a checkmark &#x2713; -->
 
<!-- Precede finished items with a checkmark &#x2713; -->
 
* Gal4-EED/luc Luciferase ICC - fixation, permeabilization, primary ab staining
 
* Gal4-EED/luc Luciferase ICC - fixation, permeabilization, primary ab staining
 +
* Gal4-EED/ luc dox induction - shipment 2 cells
  
  
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* At the primary antibody staining step, keep samples and reagents on ice overnight in the cold room
 
* At the primary antibody staining step, keep samples and reagents on ice overnight in the cold room
  
 +
 +
----
 +
'''Gal4-EED/luc dox induction'''<br>
 +
 +
Samples (Shipment 2 cells); new 6-well plate...
 +
# 0 μg/mL dox
 +
# 0.1
 +
# 0.2
 +
# 0.5
 +
# 1.0
 +
# 2.0 (induced on 6/02/13, 13 days)
 +
 +
* For this set, test earlier time points and try luc activity assay
  
  

Revision as of 18:02, 17 June 2013

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06/15/13

  • Gal4-EED/luc Luciferase ICC - fixation, permeabilization, primary ab staining
  • Gal4-EED/ luc dox induction - shipment 2 cells



Gal4-EED/luc Luciferase ICC

Samples (Shipment 1 cells)

  1. 0 μg/mL dox
  2. 0.1
  3. 0.2
  4. 0.5
  5. 1.0
  6. 2.0 (induced on 6/02/13, 13 days)
  • Harvested cells from one 6-well plate (split plate #2 for further analyses)
  • At the primary antibody staining step, keep samples and reagents on ice overnight in the cold room



Gal4-EED/luc dox induction

Samples (Shipment 2 cells); new 6-well plate...

  1. 0 μg/mL dox
  2. 0.1
  3. 0.2
  4. 0.5
  5. 1.0
  6. 2.0 (induced on 6/02/13, 13 days)
  • For this set, test earlier time points and try luc activity assay