User:Karmella Haynes/Notebook/PcTF Genomics/2013/06/12: Difference between revisions
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'''Gal4-EED/luc cells IFC'''<br> | '''Gal4-EED/luc cells IFC'''<br> | ||
* Samples: first two of wells in the 24-well plate. | |||
* Keep all others under PBS | |||
# | # 0 μg/mL dox | ||
# | # 0.1 | ||
# 0.2 | |||
# 0.5 | |||
# 1.0 | |||
# 2.0 | |||
* Use 1:200 dilution of rabbit (poly) anti-luc | * Use 1:200 dilution of rabbit (poly) anti-luc | ||
* Incubate under parafilm "covers" overnight at 4°C | |||
Revision as of 20:21, 12 June 2013
 Pc-TF Genomics
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06/12/13
Gal4-EED/luc cells IFC
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