Difference between revisions of "User:Karmella Haynes/Notebook/PcTF Genomics/2013/06/12"

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(06/12/13)
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'''Gal4-EED/luc cells IFC'''<br>
 
'''Gal4-EED/luc cells IFC'''<br>
> Samples
+
* Samples: first two of wells in the 24-well plate.
 +
* Keep all others under PBS
  
# no dox
+
# 0 μg/mL dox
# etc.
+
# 0.1
 +
# 0.2
 +
# 0.5
 +
# 1.0
 +
# 2.0
  
 
* Use 1:200 dilution of rabbit (poly) anti-luc
 
* Use 1:200 dilution of rabbit (poly) anti-luc
 +
* Incubate under parafilm "covers" overnight at 4°C
  
  

Revision as of 19:21, 12 June 2013

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06/12/13

  • Gal4-EED/luc cells IFC - stain with anti-luc overnight

Gal4-EED/luc cells IFC

  • Samples: first two of wells in the 24-well plate.
  • Keep all others under PBS
  1. 0 μg/mL dox
  2. 0.1
  3. 0.2
  4. 0.5
  5. 1.0
  6. 2.0
  • Use 1:200 dilution of rabbit (poly) anti-luc
  • Incubate under parafilm "covers" overnight at 4°C