Difference between revisions of "User:Karmella Haynes/Notebook/PcTF Genomics/2013/06/11"

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==06/11/13==
 
==06/11/13==
 
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* Gal4-EED/luc HEK +dox cytology: fix and stain cells with primary ab
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* Gal4-EED/luc HEK +dox cytology: fix and permeabilize cells
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* Use David Dreher's protocol, except dilute dry paraformaldehyde to make fresh fixing buffer.
 
* Use David Dreher's protocol, except dilute dry paraformaldehyde to make fresh fixing buffer.
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* Process all 24 wells
  
 
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Latest revision as of 20:18, 12 June 2013

Owwnotebook icon.pngPc-TF Genomics <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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06/11/13

  • Gal4-EED/luc HEK +dox cytology: fix and permeabilize cells



Gal4-EED/luc HEK +dox cytology
> Use 24-well plate from 6/10/13

  • Use David Dreher's protocol, except dilute dry paraformaldehyde to make fresh fixing buffer.
  • Process all 24 wells