User:Karmella Haynes/Notebook/PcTF Genomics/2013/01/06

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01/06/13

  • ChIP seq: run SeqMan NGen alignment on H3K27me3 mock (lane 7)
  • Expand table from 1/05/13 to include PcTF, PcTF mock, H3K27me3, H3K27me3 mock


Gene Chromosome Expected state H3K27me3
max. DOC
H3K27me3 mock
max. DOC
PcTF
max. DOC
PcTF mock
max. DOC
ALAS1 chr 3 active ~160 ~100
TBP chr 6 active ~160 ~275
ACTB chr 7 active ~180 ~1
GUSB chr 7 active ~160 ~240
PPIA chr 7 active ~90 ~480
GAPDH chr 12 active ~90 ~240
B2M chr 15 active 0 ~1
G6PD chr X (23) active ~130 0
HPRT1 chr X (23) active ~150 ~320
PGK1 chr X (23) active ~240 ~475
CDKN2A chr9 silent (H3K27me3+) ~90 ~650
MMP12 chr11 silent (H3K27me3+) ~18 ~1
MYT1 chr20 silent (H3K27me3+) ~270 ~525
OLIG1 chr21 silent (H3K27me3+) 0 0
OLIG2 chr21 silent (H3K27me3+) ~130 0